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Gene Review:

ddl - D-alanine--D-alanine ligase

Escherichia coli UTI89

Fiskus, W. et al., Fan, C. et al., Zawadzke, L.E. et al.

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Disease relevance of ddlB

The molecular structure of the D-alanine:D-alanine ligase of the ddlB gene of Escherichia coli, co-crystallized with an S,R-methylphosphinate and adenosine triphosphate, was determined by x-ray diffraction to a resolution of 2.3 angstroms [1].

High impact information on ddlB

The ddlB gene was subcloned by use of the polymerase chain reaction into an expression vector containing an optimized ribosome binding site, which expressed the DdlB enzyme to greater than 50% soluble cell protein [2].
We report the localization of three promoter regions within the ddlB-ftsA sequence of B. bacilliformis that may enhance the transcription of ftsZ mRNA [3].
The organization of the genes within the ddlB-ftsZ operon of B. bacilliformis and B. henselae (5'ddlB-ftsQ-ftsA-ftsZ 3') is similar to that of Mesorhizobium loti and Escherichia coli [3].

Biological context of ddlB

A promoter region was also identified upstream of the ddlB gene [3].

References

Vancomycin resistance: structure of D-alanine:D-alanine ligase at 2.3 A resolution. Fan, C., Moews, P.C., Walsh, C.T., Knox, J.R. Science (1994) [Pubmed]
Existence of two D-alanine:D-alanine ligases in Escherichia coli: cloning and sequencing of the ddlA gene and purification and characterization of the DdlA and DdlB enzymes. Zawadzke, L.E., Bugg, T.D., Walsh, C.T. Biochemistry (1991) [Pubmed]
Identification and characterization of the DdlB, FtsQ and FtsA genes upstream of FtsZ in Bartonella bacilliformis and Bartonella henselae. Fiskus, W., Padmalayam, I., Kelly, T., Guibao, C., Baumstark, B.R. DNA Cell Biol. (2003) [Pubmed]

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