Disease relevance of ODC

• Inhibition of ODC suggests that aminoglycoside-polyamine interactions may be an important component of the sequence of biochemical events associated with aminoglycoside toxicity [1].
• Maternal dietary protein deficiency decreases nitric oxide synthase and ornithine decarboxylase activities in placenta and endometrium of pigs during early gestation [2].
• Ornithine decarboxylase-like immunoreactive material was detected in the neurons of the Ganglion spirale and in their axonal and/or dendritic fibers [3].
• After screening 372 strains of Lactobacillus spp. isolated from a Portuguese traditional dry fermented sausage, two Lactobacillus strains, a Lactobacillus homohiochii and a L. curvatus were selected, because they were positive for tyrosine and ornithine decarboxylase activities [4].

Psychiatry related information on ODC

• The results indicate that maternal deprivation causes a marked decrease in liver ODC activity in neonatal swine as shown previously in neonatal rats (Schanberg et al., 1984) [5].
• The design of the experiment did not permit separation of the relative contributions of sensory deprivation versus food deprivation of piglets in inducing the decline in liver ODC activity [5].

High impact information on ODC

• The results show that immature granulocytes contain at least ten times more ornithine decarboxylase activity and two times more spermidine than mature granulocytes [6].
• Six hours after an inflammatory stimulus, transient increases of 14-fold and 3-fold in the activities of ornithine decarboxylase and S-adenosyl-L-methionine decarboxylase, respectively, were observed in immature bone marrow granulocytes [6].
• The concomitant induction of ornithine decarboxylase activity is insensitive to indomethacin inhibition [7].
• The nonpromoting derivative 4-O-methyl-12-O-tetradecanoylphorbol-13-acetate evokes a prostaglandin-independent, i.e., indomethacin-insensitive, stimulation of cell proliferation without inducing ornithine decarboxylase activity [7].
• In both segments, abrupt increases in DNA mass, crypt depth, ornithine decarboxylase activity, and crypt cells in S-phase occurred between enteral intakes of 40% and 60% [8].

Biological context of ODC

• The kinetics of this ODC response were investigated at intervals including, 2, 4.5, 12, 18, 24 and 72 hr after tape stripping [9].
• Therefore, ODC activity was inhibited by epidermal polyamine-dependent protein kinase-mediated phosphorylation [10].
• From these results it is suggested that the uptake of Na(+)-dependent cotransported sugars increase ODC activity by enhancing ODC gene transcription and that this process may be dependent on cell volume expansion [11].
• At d 40 or 60 of gestation, they were hysterectomized, and placenta and endometrium were obtained for incubations, NOS and ODC assays, and measurements of free amino acids and polyamines [2].
• Enzymatic activity was defined as the rate of decarboxylation of [14C]ornithine sensitive to a specific ODC inhibitor, alpha-difluoromethylornithine (DFMO) [1].

Anatomical context of ODC

• The results of this study are consistent with the concept that cAMP can mediate gonadotropin stimulation of ODC in porcine granulosa cells [12].
• The present study examines the effect of alpha MDG on LLC-PK1-cell ODC mRNA expression [11].
• Under defined conditions in vitro FSH and LH caused a dose-dependent stimulation of ornithine decarboxylase (ODC) activity in granulosa cells isolated from porcine ovarian follicles [13].
• A 80 KD polypeptide of ODC comigrated with the polypeptide of cytosol which was phosphorylated by polyamine-dependent protein kinase [10].
• At 29 days of age, pigs were used to prepare jejunal enterocytes for ODC assay and metabolic studies [14].

Associations of ODC with chemical compounds

• Actinomycin D was also a potent inhibitor of ODC mRNA expression by alpha MDG [11].
• However, in time-course experiments, progesterone secretion increased significantly before the rise of ODC activity [15].
• Cycloheximide had very little effect on the ability of this sugar to increase ODC mRNA [11].
• The administration of saturating concentrations of LH, prostaglandin E2, L-epinephrine, or 8-bromo-cAMP each elicited highly significant increases in progesterone production, which correlated closely with corresponding stimulated levels of ODC activity [15].
• Non-metabolizable analogues of glucose, including 1-O-methyl alpha-D-glucopyranoside (alpha MDG), that are co-transported with Na+ increase the specific activity of ornithine decarboxylase (ODC) in LLC-PK1 cells [Lundgren and Vacca (1990) Am. J. Physiol. 259, C647-C653] [11].

Other interactions of ODC

• N,O'-Dibutyryl cAMP (1.0--10.0 mM) caused a dose-dependent stimulation of ODC activity which equaled the maximal effect of LH but was significantly less effective than the saturating dose of FSH [12].
• Thus, of the three organs studied, only liver responded to menhaden oil with changes in both ODC itself or some of its metabolic engendered products and thymidine kinase; at least for one of the parameters, ODC, change associated with dietary MO was dependent on whether the swine were preloaded with cholesterol [16].
• Placentae as well as amniotic and allantoic fluids were analyzed for arginase, proline oxidase, ornithine aminotransferase (OAT), ornithine decarboxylase (ODC), proline transport, concentrations of amino acids and polyamines, and polyamine synthesis using established radiochemical and chromatographic methods [17].

Analytical, diagnostic and therapeutic context of ODC

• Northern blot analysis indicated that SSAT mRNA levels change with distinct pregnancy stages, in contrast to those for the PA biosynthetic enzyme ornithine decarboxylase (ODC) [18].
• The ornithine decarboxylase polyamine pathway is activated in the nervous system following axotomy or denervation [19].

References