Psychiatry related information on B-G

• Subgrouping the Bolivian and Indian indigenous hens into monomorphic BG populations revealed individual differences based on the B-types [1].

High impact information on B-G

• The chicken major histocompatibility complex (MHC) is commonly defined by serologic reactions of erythrocytes with antibodies specific to the highly polymorphic MHC class I (BF) and MHC class IV (BG) antigens [2].
• B homozygotes identified serologically were compared by B-G genotyping using restriction fragment length polymorphism analysis [3].
• These repeats could explain the bewildering variation in size of B-G proteins within and between haplotypes [4].
• We used rabbit antisera to the chicken MHC erythrocyte molecule B-G and to the class I alpha chain (B-F) to screen lambda gt11 cDNA expression libraries made with RNA selected by oligo-dT from bone marrow cells of anemic B19 homozygous chickens [4].
• The B-G antigens are highly polymorphic antigens encoded by genes located within the major histocompatibility complex (MHC) of the chicken, the B system [5].

Biological context of B-G

• Isolation of a cDNA clone from the B-G subregion of the chicken histocompatibility (B) complex [5].
• One clone chosen for further study, lambda bg28, was confirmed as a B-G subregion cDNA clone by the results obtained through using it as a nucleic acid hybridization probe [5].
• Southern blot analyses of the hybridization of lambda bg28 with genomic DNA from birds of known haplotypes strongly suggest that the B-G antigens are encoded by a highly polymorphic multigene family [5].
• The microsatellite marker LEI0258 is known to be physically located within the MHC, between the BG and BF regions [2].
• The humoral response in another pair of recombinant haplotypes originating in 2 different White Leghorn chickens being BF21, BL21, BG15 (BR4) and BF15, BL15, BG21 (BR5) on the MHC locus indicated that the BG locus may perform an adjuvant effect on the antibody response as well [6].

Anatomical context of B-G

• The B-G antigens of the chicken MHC are found only on erythrocytes and correspond to neither MHC class I nor class II antigens [5].
• Several clones were selected from a lambda gt11 erythroid cell expression library by means of rabbit antisera prepared against a purified, denatured B-G antigen [5].
• The humoral response of 1 pair of recombinants isolated from a Red Jungle Fowl (BW3 and BW4) being identical on BF and BG, but different on BL, indicated that part of the primary vaccine response was an MHC II restricted T-cell dependent response [6].

Analytical, diagnostic and therapeutic context of B-G

• Within the White Leghorn line there were no discrepancies between serotypes and typing patterns for B-G by either IEF or SDS-PAGE [7].
• Biochemical analysis involved immunoprecipitations from radiolabelled cells using monoclonal antibodies recognizing monomorphic determinants of B-F (F21-2) or B-G (18-6G2) followed by analysis on SDS-PAGE and/or IEF [7].

References