Disease relevance of Hex-C

• The phenotypes in myotonic dystrophy types 1 and 2 (DM1 and DM2) are similar, suggesting a shared pathophysiologic mechanism [1].

High impact information on Hex-C

• Three band-morph mutants, one at the Mdh locus, one at the Hex-C locus, and one at the Amy locus, were detected out of 1,658,308 allele replications [2].
• Antisera against purified Hex A and Hex C were raised in rabbits [3].
• In D. melanogaster latitudinal clines are seen for amino acid polymorphisms at the Hex-C and Hex-t2 loci [4].
• As part of a larger study contrasting patterns of variation in regulatory and nonregulatory enzymes of the central metabolic pathways we have examined the molecular variation in four uncharacterized hexokinase genes unique to muscle, fat body, and testis in Drosophila melanogaster, D. simulans, and D. yakuba [4].
• Exposure to benzaldehyde at days 2-5 of adult life, but not at 8-11, causes behavioral adaptation as well as structural changes in DM2 and V glomeruli [5].

Biological context of Hex-C

• Amino acid sequence alignment of DM1 and DM2 shows approximately 45% homology with yeast and human hexokinases [6].
• Southern blot analysis suggests that the D. melanogaster genome contain a single copy of DM1 and DM2 sequences [6].
• Such sexually mosaic flies courted not only females but also males when the DM2, DA3 and DA4 glomeruli were feminized, indicating that these substructures in the antennal lobe may be involved in the determination of the sexual orientation of flies [7].

Anatomical context of Hex-C

• Hexokinase coding DM1 and DM2 sequences were obtained from genomic DNA of a Drosophila melanogaster cell line by PCR amplification strategy [6].

Associations of Hex-C with chemical compounds

• The analog was also found to be a substrate for all of the enzymes tested, including creatine kinase, pyruvate kinase, hexokinase, and myosin, with values of Km and Vmax that were within a factor of 10 of those for ATP [8].
• Drosophila melanogaster and Ceratitis capitata are insensitive to mannose and have excess of mannosephosphate isomerase over hexokinase [9].

Analytical, diagnostic and therapeutic context of Hex-C

• Expression levels for DM1 and DM2 encoded message were found to be similar in different stages of development as seen by RT-PCR [6].
• Major forms of hexokinases (Hex A and Hex C) in Drosophila melanogaster were purified to homogeneity by utilizing affinity chromatography and preparative isoelectric focusing column as the key steps [3].

References