High impact information on ImpE1

• In addition, we find that dib mutant embryos have very low titers of ecdysone and 20-hydroxyecdysone (20E) and fail to express IMP-E1 and L1, two 20E-inducible genes, in certain tissues of the embryo [1].
• Like dib, mutations in the spook (spo) locus result in low embryonic ecdysteroid titers, severe late embryonic morphological defects, and a failure to induce IMP-E1 [1].
• Induction studies in the presence of cycloheximide establish that IMP-E1 is a primary response locus while IMP-L1 transcription is a secondary response [2].
• Differences between the induction characteristics of IMP-E1 and the early 20-hydroxyecdysone-responsive gene E74 lead us to propose an addition to the Ashburner model for the 20-hydroxyecdysone regulatory hierarchy [2].
• The distribution of transcripts is consistent with the hypothesis that the IMP-E1 gene product is involved in the cell rearrangements associated with morphogenesis of the disc epithelium [3].

Anatomical context of ImpE1

• Within the imaginal leg disc, the IMP-E1 transcript is expressed more abundantly in the proximal than in the distal portions of the epithelium [3].
• The IMP-E1 transcript is also expressed at pupariation in glial cell layers that ensheath the brain [3].
• Genes expressed during imaginal disc morphogenesis: IMP-E1, a gene associated with epithelial cell rearrangement [3].

References