Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Editor

Personal info

View

About

Terms

Javascript disabled

Please enable Javascript support in your browser to use this application.

Instructions on how to enable JavaScript on different browsers can be found here: http://www.google.com/support/bin/answer.py?answer=23852.

[edit this page]

Gene Review:

IL18 - interleukin 18 (interferon-gamma-inducing...

Felis catus

Ishizaka, T. et al., Dunham, S.P. et al., Foley, J.E. et al., O'Donovan, L.H. et al.

Foley, Rand, Leutenegger, O'Donovan, McMonagle, Taylor, Bain, Pacitti, Golder, McDonald, Hanlon, Onions, Argyle, Jarrett, Nicolson, Dunham, Flynn, Rigby, Macdonald, Bruce, Cannon, Golder, Hanlon, Harbour, Mackay, Spibey, Jarrett, Neil,

Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of IL18

Of six kittens immunised with FeLV DNA and IL-18, all were protected from viraemia and five from latent infection [1].
To test the ability of these multi-gene vaccines to protect against two feline immunodeficiency virus (FIV) isolates of differing virulence, cats were immunized using either DNA vaccine alone or co-administered with interleukin-12 (IL-12) and/or interleukin-18 (IL-18) cytokine DNA [2].

High impact information on IL18

IL-12 and IL-18 constructs were modified to ensure effective expression, and bioactivity was demonstrated using specific assays [1].
The predicted amino acid sequence of feline IL-18 cDNA showed 77.2, 84.8, 60.2 and 62.6% similarity with those of human, dog, rat and mouse counterparts, respectively [3].
The nucleotide sequence of feline IL-18 cDNA obtained in this study was 712bp long and contained its entire open reading frame encoding 192 amino acid residues [3].
Expression of IL-18 mRNA was detected in various tissues including spleen, liver and cerebrum in the cat [3].
Interleukin-18 (IL-18) is a cytokine with potent interferon-gamma-inducing activity, and plays an important biologic role in the enhancement of the activity of natural killer cells and cytotoxic T-lymphocytes [3].

Biological context of IL18

IL-1 beta, IL-6, IL-12, IL-18, TNF-alpha, macrophage inhibitory protein (MIP)-1 alpha, and RANTES showed no upregulation in the brains of control cats, moderate upregulation in neurological FIP cats, and very high upregulation in generalized FIP cats [4].

References

A vector expressing feline mature IL-18 fused to IL-1beta antagonist protein signal sequence is an effective adjuvant to a DNA vaccine for feline leukaemia virus. O'Donovan, L.H., McMonagle, E.L., Taylor, S., Bain, D., Pacitti, A.M., Golder, M.C., McDonald, M., Hanlon, L., Onions, D.E., Argyle, D.J., Jarrett, O., Nicolson, L. Vaccine (2005) [Pubmed]
Protection against feline immunodeficiency virus using replication defective proviral DNA vaccines with feline interleukin-12 and -18. Dunham, S.P., Flynn, J.N., Rigby, M.A., Macdonald, J., Bruce, J., Cannon, C., Golder, M.C., Hanlon, L., Harbour, D.A., Mackay, N.A., Spibey, N., Jarrett, O., Neil, J.C. Vaccine (2002) [Pubmed]
Molecular cloning of feline interferon-gamma-inducing factor (interleukin-18) and its expression in various tissues. Ishizaka, T., Setoguchi, A., Masuda, K., Ohno, K., Tsujimoto, H. Vet. Immunol. Immunopathol. (2001) [Pubmed]
Inflammation and changes in cytokine levels in neurological feline infectious peritonitis. Foley, J.E., Rand, C., Leutenegger, C. J. Feline Med. Surg. (2003) [Pubmed]

Contributions to this collaborative article are from individual authors of WikiGenes or mined by the WikiGenes Data Mining Engine from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.About WikiGenes Open Access Licence Privacy Policy Terms of Use apsburg