High impact information on GAPDH

• This study demonstrates that acrylamide inhibits GAPDH in Schwann cells [1].
• MnBK and 2,5-HD both inhibited GAPDH and PFK activities selectively. n-Hexane and 2-hexanol had no effect on GAPDH and PFK activities; 5-hydroxy-2-hexanone and 2,5-hexanediol exhibited a slight inhibitory effect on these enzymes [2].
• The effect of n-hexane, 2-hexanol, 5-hydroxy-2-hexanone, 2,5-hexanediol, methyl n-butyl ketone ( MnBK ) and 2,5-hexanedione (2,5-HD) has been studied in vitro on crystalline glyceraldehyde-3-phosphate dehydrogenase (GAPDH), DL-glyceraldehyde-3-phosphate: NAD oxidoreductase (phosphorylating) EC [2].
• The etiology of acrylamide neuropathy: enolase, phosphofructokinase, and glyceraldehyde-3-phosphate dehydrogenase activities in peripheral nerve, spinal cord, brain, and skeletal muscle of acrylamide-intoxicated cats [3].
• GAPDH mRNA was readily detectable in cDNA prepared from short-time cultivated peripheral blood monocytes [4].

Biological context of GAPDH

• Messenger RNA (mRNA) from the universally expressed housekeeping gene GAPDH proved to be useful as an amplification control and allowed for correction of variations in the efficiencies of RNA extraction and reverse transcription [5].

Associations of GAPDH with chemical compounds

• The significance of GAPDH inhibition by acrylamide in denervated nerves and its relation to distal axonopathy has been discussed [1].
• Thiol agarose and glyceraldehyde-3-phosphate dehydrogenase were blocked with methylmercury or p-mercuribenzoate [6].

Analytical, diagnostic and therapeutic context of GAPDH

• An integrated density value (IDV) was determined by use of densitometry, and the IDV ratio for enFeLV to GAPDH was calculated for each enFeLV primer set [7].
• The density of each RT-PCR product band for enFeLV was compared with that of a constitutively expressed gene, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) [7].

References