Disease relevance of PDCD2

• BCL6 deregulation may lead to persistent down-regulation of PDCD2, reduced apoptosis, and, as a consequence, accumulation of BCL6-containing lymphoma cells [1].
• The RT-PCR products (AY948416, AY948417) of PDCD2 from RNA of human embryonic kidney 293T (HEK293T) and gastric cancer AGS cell line lost common 99 bp when compared with the sequences of NCBI database (NM_002598, NM_144781) [2].

High impact information on PDCD2

• These results raise the possibility that BCL6 may regulate apoptosis by means of its repressive effects on PDCD2 [1].
• We have shown that the highly conserved C-terminal WYF domain of HCF-1 protein interacts with the MYND domain of the PDCD2 protein [3].
• This analysis suggested the gene PDCD2 originally thought to be involved in programmed cell death to be probably down-regulated in malignant B-cell lymphomas compared to normal B lymphocytes [4].
• Nevertheless, mutation analyses failed to identify mutations in the coding region of PDCD2 in nine lymphomas with FISH-proved 6q27 deletions [4].
• Furthermore, epigenetic studies in these nine and an additional 48 lymphomas did not show altered methylation of the PDCD2 locus in these tumors [4].

Biological context of PDCD2

• The A allele of the G/A1180 single nucleotide polymorphism (SNP) at the PDCD2 gene, which was significant in its preferential transfer from parents to diabetic children (75 transmissions vs. 47 non-transmissions, chi2=12.85, P corrected=0.0038), was found to be associated with T1D [5].
• We searched GEO (Gene Expression Omnibus) about PDCD2 and MGC13096 [2].
• There are only two isoforms of PDCD2 and MGC13096 containing PDCD2(C) domain in human genome [2].
• This cDNA, termed PDCD2 (programmed cell death-2), contained an open reading frame of 1032 nucleotides encoding 344 amino acids; it revealed 81% identity in DNA sequence and 83% identity in amino acid sequence with rat Rp8 [6].
• In a Transwell migration test, ANP also increased the cell migration, and RP8, wortmannin, and PD-98059 blocked this increase [7].

Anatomical context of PDCD2

• PDCD2 is detectable in cells of the germinal center in areas where there is less BCL6 expression as well as in the mantle zone, where there is little or no BCL6 expression [1].
• PDCD2 (NM_002598) was over expressed when endothelial cells treated with leukotriene D4 or natural killer cells were activated by IL-2 [2].

Associations of PDCD2 with chemical compounds

• PDCD2 is a negative regulator of HCF-1 (C1) [3].
• The TBP- and PDCD2-encoding genes are linked also in Drosophila, and, together with proteasomal subunit C5 gene, they are syntenic in human, mouse, C. elegans, and Schizosaccharomyces pombe [8].
• An extraction procedure from plasma samples was developed on Bond Elut RP8 500-mg cartridges; conditioning was performed with 5 ml methanol and 5 ml water, after which 1 ml plasma that had previously been spiked with 5 microM S-Ag was applied [9].
• A 10-cm X 2-mm i.d., 5-micrometers Lichrosorb RP8 column with acetonitrile-0.1 M citric acid as the eluent was used [10].
• A new HPLC separation is described that efficiently resolves all four compounds employing gradient elution with 10 mM ammonium acetate, increasing methanol (20-80% over 15 min), and a 15-cm by 3-mm Symmetry Shield (RP8) column [11].

Other interactions of PDCD2

• The orthologs of tightly linked C. elegans genes, coding for BRP44L and PDCD2, map to about 2-Mb interval on human region 6q27 and on mouse Chr 17 [8].
• Immunohistochemistry reveals the anticipated inverse relationship between BCL6 and PDCD2 expression in human tonsil [1].
• G/A1180 dimorphism and two other SNPs, C/T771 TBP and G/T(-271) PDCD2, were shown to share three common haplotypes, two of which (A-T-G and A-T-T) have been associated with higher development risk of T1D [5].
• These findings suggest that the PDCD2 gene is a likely susceptibility gene for T1D within IDDM8 [5].

Analytical, diagnostic and therapeutic context of PDCD2

• We assigned the PDCD2 locus to chromosomal band 6q27 by fluorescence in situ hybridization (FISH) [6].
• The UV spectra and relative retention times (RRT) of 2682 toxicologically relevant substances were measured by high-performance liquid chromatography with diode array detection (HPLC-DAD) in an acetonitrile phospate buffer (pH 2.3) mixture on an RP8 column and were arranged in a database [12].

References