High impact information on LOC538404

• The structure of the sarcomeric M band: localization of defined domains of myomesin, M-protein, and the 250-kD carboxy-terminal region of titin by immunoelectron microscopy [1].
• Myomesin also seems to bridge the central M1-line and is oriented parallel to the long axis of the myofibril [1].
• We report a method for isolating homogeneous myomesin from mammalian skeletal muscle [2].
• Reactivity of the antibody on stable proteolytic fragments of myomesin showed that the phosphorylation site must reside within the carboxy-terminal 60 residues [2].
• Striations in the AV node were less evident than in the AV bundle and the bundle branches in sections incubated with antibodies against myomesin as well as against MM-creatine kinase [3].

Anatomical context of LOC538404

• The differentiation of Purkinje fibres and ordinary ventricular and atrial myocytes in bovine hearts was studied with specific antibodies against M-line proteins (MM-creatine kinase and myomesin) and with enzyme histochemistry (succinate dehydrogenase and mitochondrial glycerol-3-phosphate dehydrogenase) [4].

Analytical, diagnostic and therapeutic context of LOC538404

• Electron microscopy of myomesin revealed short flexible rods with a molecular length of about 50 nm [2].
• The M-line proteins, myomesin and MM-creatine kinase, were detected earlier, by means of immunohistochemistry, in the AV bundle and bundle branch cells than in the AV node cells [3].
• Analysis by confocal laser microscopy of CM-stimulated myocytes showed actin staining throughout the whole cell body up to the peripheral extensions, with concomitant appearance of myomesin in a cross-striated pattern [5].

References