Disease relevance of F12

• When tested in vivo for suppression of delayed-type hypersensitivity to ABA, two of these cell lines, A4 and F12, were shown to produce suppressive supernatant factors [1].
• We have developed a serum-free, chemically defined growth medium containing casein, insulin, transferrin, testosterone, and linoleic acid in Dulbecco's modified Eagle's medium/Ham's F12 medium, 1:1 (vol/vol), for growing murine T lymphomas [2].
• Freshly disaggregated mastocytoma cells were cultured in Dulbecco's modified Eagle's medium (DME)-H16 mixed with 50% Ham's F12 and supplemented with histidine and 5% allergic dog serum (ADS) [3].
• In two preceding papers we described the cloning of two astrocytic cell lines by simian virus 40 (SV40) transformation of embryonic mouse mesencephalon (F7-Mes) and striatum (F12-Str) [4].
• F12 MAbs were used for immunoaffinity purification of BoIFN-alpha, and recombinant BoIFN-alpha C from Escherichia coli extracts was purified to homogeneity in SDS-PAGE analysis and to a specific activity of 2 X 10(8) U/mg with 90% recovery of activity [5].

High impact information on F12

• This cloned suppressor cell line, F12, produces a culture supernatant factor that is suppressive at dilutions up to 1:100 and has provided material for genetic and immunochemical analysis [1].
• Fluorescence analysis of the F12 cells with appropriate antisera demonstrated this T cell hybrid to be Thy 1.2+, Lyt 1+,2-, and surface immunoglobulin negative, the surface marker phenotype of conventional ABA-specific suppressor T cells [1].
• Sertoli cells cultured in F12/DME were pulse-labeled with 131I-FSH for 10 min at 4 degrees C, followed by cold chase for various periods of time [6].
• Biologic activities and immunochemical characteristics of an azobenzenearsonate- (ABA)specific suppressor T cell factor produced by a longterm T cell hybridoma, F12, were studied [7].
• These results demonstrated that F12 is a functioning hybrid cell line of the first-order suppressor T cell subset [7].

Biological context of F12

• F20 generation breeding pairs from the nine surviving strains and an F12 pair from the extinct line were genotyped at 319 genetic markers (primarily microsatellites) spanning most of the genome [8].
• The results showed that maximum viable cell densities in eRDF medium were up to 3-times higher than in RPMI and maximum Ig titres were 2-8-times higher than in DMEM/F12 and RPMI [9].
• However, in the presence of HEPES as well as in F12-medium with HEPES, taurine restored granule cell migration [10].

Anatomical context of F12

• The survival of unstimulated murine T cells in this Iscove/F12 medium is poor, but B cells survive for relatively long periods of time [11].
• In vivo administration of F12 culture supernatant resulted in the suppression of ABA-specific delayed-type hypersensitivity (DTH) responses and the inhibition of priming for ABA-specific cytotoxic T lymphocyte responses [7].
• Following a thorough wash to remove liberated cells, the remaining cementum fragments were plated in Dulbecco's modified Eagle's medium/F12 medium containing 10% fetal bovine serum [12].
• Optimal culture conditions depend on both the age of the animal and the type of muscle explanted, but the majority of skeletal muscle explants produce large numbers of satellite cells within 4-10 days of explanting when cultured in Dulbecco's modified Eagle's medium/Ham's F12 medium supplemented with high levels of fetal calf serum (10-20%) [13].
• NPCs from E12.5 rat ventral mesencephalon were cultured as neurospheres in DMEM/F12 medium containing N2 supplements and bFGF [14].

Associations of F12 with chemical compounds

• MATERIALS AND METHODS: Pancreatic ductal cells were grown in serum-free DMEM/F12 medium in the presence of cholera toxin or 8-bromo-cyclic adenosine monophosphate, which is known to be an intracellular cAMP generator [15].
• Few newborn DRG neurons attached on polylysine coated coverslips in Ham's F12 medium, although most adult neurons attached on them [16].
• Mouse motoneurons were isolated from dissociated E15 mouse spinal cord and grown on polyornithine-coated round coverslips in a growth medium (DMEM/F12) supplemented with progesterone, trans-ferrin, selenium, horse serum and muscle extract [17].
• J774A.1 cells cultured in RPMI exhibit a 5-fold increase in nitrites in culture supernatants after LPS stimulation whereas those in DMEM/F12 do not [18].
• Mice belonging to F8, F12, F14 and F20 generation of a multigeneration study reared on 20% (v/v) ethanol in water as the sole drinking source were investigated for their immune competence using various parameters [19].

Other interactions of F12

• F11 which is the richest in PLA2 activity is less toxic than F3, which contains a small amount of PLA2, and F12 is the lowest in lethality and PLA2 activity [20].
• After 24 h, the medium was replaced with DME/F12 degrees supplemented with SGF, EGF, 1, or Tr, in two-, three- or four-way combinations [21].
• No difference in growth rate was observed between the two in DMEM/F12, although MT (-/-) cells displayed a 6-fold decrease in p27(Kip1), a two fold increase in p53 and a slight increase in p21(Waf1) [22].
• Improved survival and differentiation of newborn and adult mouse neurons in F12 defined medium by fibronectin [16].
• When the growth of granule cells from cerebella of staggerer mutant mice is investigated in monolayer cell cultures using modified Hams F12 medium plus fetal calf serum, cells from the mutant are found to clump less and survive longer than their wild-type counterparts [23].

Analytical, diagnostic and therapeutic context of F12

• While F11 is closer to F2 less than 10 kDa, F12 has a molecular weight around 13.7 kDa, as resulted after applying the SDS-PAGE procedure [24].
• We isolated motoneurons from E15 dissociated mouse spinal cord by density centrifugation and planted them onto poly-ornithine-coated coverslips in a growth medium (DMEM/F12) supplemented with progesterone, transferrin, selenium, horse serum and muscle extract [25].

References