High impact information on Slit1

• The neuronal repellent Slit inhibits leukocyte chemotaxis induced by chemotactic factors [1].
• Here we report that Slit, a secreted protein previously known for its role of repulsion in axon guidance and neuronal migration, can also inhibit leukocyte chemotaxis induced by chemotactic factors [1].
• Conversely, inhibition of Slit-Robo interactions by Robo-Fc fusion proteins or by a dominant-negative Robo attenuates dendritic branching [2].
• Regulation of cortical dendrite development by Slit-Robo interactions [2].
• A basic conservation of cell migration guidance mechanisms in the nervous and immune systems was proposed when Slit, known for its role in axon guidance, was found to inhibit chemokine-induced leukocyte chemotaxis in vitro [3].

Biological context of Slit1

• The splicing product, Slit1alpha, arises through alternative splicing at the C-terminus of Slit1, causing defects in the cysteine knot domain [4].
• Thus, the results identify that Drm and Dan can interact with Slit proteins and act as inhibitors of monocyte chemotaxis, demonstrating a previously unidentified biological role for these proteins [5].
• Robo, the receptor for the midline repellent Slit, is a member of the cell adhesion molecule (CAM) Ig superfamily [6].

Anatomical context of Slit1

• To identify Slit-responsive neurons and to help analyze Slit function, we have studied, by in situ hybridization, the expression pattern of slits and their receptors robo1 and robo2, in the rat central nervous system from embryonic stages to adult age [7].
• We have analyzed the role of the Slit family of repellent axon guidance molecules in the patterning of the axonal projections of retinal ganglion cells (RGCs) within the embryonic rat diencephalon and whether the slits can account for a repellent activity for retinal axons released by hypothalamus and epithalamus [8].
• Changes in mRNA of Slit-Robo GTPase-activating protein 2 following facial nerve transection [9].
• In situ hybridization studies indicated that the rat Slit-1 mRNA is specifically expressed in the neurons of fetal and adult forebrains [10].
• Northern blot analysis revealed that the human Slit-1, -2, and -3 mRNAs are exclusively expressed in the brain, spinal cord, and thyroid, respectively [10].

Associations of Slit1 with chemical compounds

• RESULTS: Slit lamp microscope observations indicated that curcumin at 0.002% (group C) delayed the onset and maturation of cataract [11].

Regulatory relationships of Slit1

• It was reported that the signal from Robo is transmitted to a specific family of GTPase-activating proteins (GAPs) named Slit-Robo GAPs [9].

Other interactions of Slit1

• Extracellular Ig domains 1 and 2 of Robo are important for ligand (Slit) binding [6].

Analytical, diagnostic and therapeutic context of Slit1

• Our results suggest that srGAP2, as a part of Slit-Robo pathway, plays an important role in the axonal regeneration after axotomy [9].

References