High impact information on Slc9a3r2

• Targeted disruption of the mouse NHERF-1 gene eliminated NHERF-1 expression in kidney and other tissues of the mutant mice without altering NHERF-2 levels in these tissues [1].
• By using immunohistochemistry combined with light and electron microscopy, we found that many populations of astrocytes, as well as some populations of neurons, were immunopositive for NHERF-2 throughout the mouse brain [2].
• Several proteins known to interact with NHERF-2 are abundantly expressed in the central nervous system, but little is known about NHERF-2 localization in the brain [2].
• NHERF-2 immunostaining was associated mainly with the plasma membrane of neurons and astrocytes [2].
• However, NHERF-2 immunoreactivity was also observed in association with synaptic vesicles in putative glutamatergic axon terminals [2].

Anatomical context of Slc9a3r2

• Although NHERF-2 can be detected weakly in the microvilli, it is expressed predominantly at the base of the microvilli in the vesicle-rich domain [3].
• Using the farwestern protein-blotting technique and recombinant fusion proteins containing the Sry Q-rich domain as probes, three Sry interactive proteins of 94, 32 and 28 kDa apparent molecular weight (Sip-1, -2 and -3 respectively) were consistently detected in adult testis [4].
• This study reports production of somites with roughly half rostro-caudal length in homozygous Sip1 (Smad-interacting protein 1) knockout mouse embryos [5].
• Quantitative analysis of the subcellular distribution of NHERF-2 immunostaining in four brain structures, cerebral cortex, hippocampus, striatum, and cerebellar cortex, showed that NHERF-2 was expressed mainly in astrocytic processes but was also sometimes observed in both pre- and postsynaptic neuronal elements [2].

Physical interactions of Slc9a3r2

• In expression systems and in yeast, Na/H exchanger regulatory factor (NHERF)-1 and NHERF-2 have been demonstrated to interact with the renal brush border membrane proteins NHE3 and Npt2 [3].

Analytical, diagnostic and therapeutic context of Slc9a3r2

• Full-length mGluR5 robustly associated with full-length NHERF-2 in cells, as assessed by co-immunoprecipitation and confocal microscopy experiments [6].

References