Disease relevance of Nde1

• Ablation of the LIS1-interacting protein Nde1 (formerly mNudE) in mouse produces a small brain (microcephaly), with the most dramatic reduction affecting the cerebral cortex [1].
• Human equivalent of the mouse Nude/SCID phenotype: long-term evaluation of immunologic reconstitution after bone marrow transplantation [2].
• We inoculated Nude mice with DA virus to clarify the role of immune-mediated demyelination [3].
• Bcl-xL gene induces metastasis in the lung, lymph nodes and bone when breast cancer cells are inoculated in Nude Balb/c mice [4].
• To help elucidate the role of host immune mechanisms involved in demyelination, we studied TMEV infection in Nude mice [5].

Psychiatry related information on Nde1

• A stimulation of locomotor activity was evidenced in all tested strains of mice: Swiss CD1, BALB/c, DBA2, C3H, B6CBAF1/JICO, Nude CD1 and in all tested strains of rats: Long Evans, Sprague Dawley, Wistar [6].

High impact information on Nde1

• In vitro analysis demonstrated that Nde1 is essential for centrosome duplication and mitotic spindle assembly [1].
• BrdU birthdating revealed retarded and modestly disorganized neuronal migration; however, more dramatic defects on mitotic progression, mitotic orientation, and mitotic chromosome localization in cortical progenitors were observed in Nde1 mutant embryos [1].
• In contrast, Nude/+ mice raised in a pathogen-free colony had no detectable circulating MHV antibodies and generated a primary antibody response which gradually increased to peak levels 14 to 28 days after infection [7].
• This study concerns the antigenic competition among heterologous red cells, a T suppressor-dependent phenomenon, in three cases, when the two antigens are administered by various routes, in (a) splenectomized mice or in Nude mice reconstituted with (b) lymph node cells, or spleen cells [8].
• METHODS: Nude mice bearing tumours containing human 293 cells were grafted with specific pathogen-free pig islets or PERV-producing pig PK15 cells to determine whether pig cells could transmit PERV to mouse and human cells in vivo [9].

Chemical compound and disease context of Nde1

• Using orthotopically implanted mouse Hepa 1-6 hepatoma in the liver of Nude mice, the antitumor effect of Bamet-UD2 was compared with that of a previously characterized compound of this family, Bamet-R2 [cis-diamminebis-ursodeoxycholate platinum(II)], and cisplatin [10].
• Nuclear Trafficking of a Gadolinium Conjugate in Nude Mice Xenografted with Human LN-229 Glioma [11].
• METHODS: Nude mice bearing AGS, Hs 746T, and NCI-N87 human gastric carcinomas were treated with AN-238, AN-215, or their cytotoxic moiety 2-pyrrolinodoxorubicin (AN-201) [12].
• MATERIALS AND METHODS: Nude mice bearing subcutaneous human head and neck squamous cell carcinoma xenografts were treated with single intralesional (IL) injections of tumor-reactive human monoclonal IgM (CR4E8) labeled with 25-394 microCi of yttrium-90 (90Y) [13].
• Effect of Ascorbic Acid, Lysine, Proline, and Green Tea Extract on Human Osteosarcoma Cell Line MNNG-HOS Xenografts in Nude Mice: Evaluation of Tumor Growth and Immunohistochemistry [14].

Biological context of Nde1

• Using LacZ reporter gene activity, we detected efficient transduction in all mouse strains (Nude, mean 11%, range 4.2-21%; C57BL/10, mean 12%, range 3.4-20%; Nude mdx, mean 4.3%, range 2.1-7% at 4 weeks post-injection and 6.6%, range 1.3-12% at 12 weeks post-injection) [15].
• The significance of the early development of autoimmunity and glomerular lesions in Nude mice is discussed [16].
• For in vivo assays, the transfection level in tumors induced in Nude mice has been optimized by studying the effects of charge ratio, of the helper lipid and of the injection volume [17].
• Wound healing in the ears of Athymic Nude-nu mice resembles regeneration [18].
• The present report demonstrates differential DNA-repair activity among 14 strains of immature (20 +/- 2 days old) male mice (inbred strains: C57BL/6J, RF/J, Nude homo/nu, RIII/2J, PL/J, AKR/J, Nude hetero/nude, C3H/HeJ, SWR/J, SM/J, ST/J, LP/J, BALB/cJ and random-bred strain: CD-1) [19].

Anatomical context of Nde1

• While there may be other defects in Nude mice that might contribute to the effects shown here, these data suggest that the brief influx of T cells in this model of chemically-induced demyelination could play a role in macrophage/microglial activation and demyelination [20].
• In the present study, we examined whether this brief influx of T cells contributes to the activation of macrophages/microglia and demyelination by injecting LPC into the dorsal column white matter of athymic Nude mice that lack T cells [20].
• We show that there is a significant reduction in macrophage/microglial activation and myelin clearance after LPC injection in Nude mice as compared with wildtype controls [20].
• The studies indicate that in Nude mice TMEV causes a lytic infection of oligodendrocytes producing demyelination independent of the T lymphocyte immune system [5].
• In ciliated cells of thymic cysts in Nude mice, ciliary rootlets are constantly and closely related to smooth endoplasmic reticulum and clear vesicles [21].

Associations of Nde1 with chemical compounds

• Treatment with BANB-6, but not cisplatin, was able to prolong the life span of Nude mice bearing tumors formed by Hepa 1-6/R cells orthotopically implanted in the liver [22].
• METHODS AND MATERIALS: Nude mice bearing Dunning prostate tumors (R3327-AT) were injected with (18)F-FDG and pimonidazole, bromodeoxyuridine, and, 1 min before sacrifice, with Hoechst 33342 [23].
• Further, bone marrow cells from Nude mice failed to produce high levels of histamine following alloantigen stimulation [24].
• Antitumoural Effects of the Pyridyl Cyanoguanidine CHS 828 on Three Different Types of Neuroendocrine Tumours Xenografted to Nude Mice [25].
• After termination of treatment with sulfadiazine the Nude mice gradually died, whereas the normal hairy mice survived [26].

Analytical, diagnostic and therapeutic context of Nde1

• METHODS AND MATERIALS: Nude mice with human glioma xenograft tumors were treated with fractionated local tumor radiation of 250 cGy/fraction/day and 5 mg/kg ATO for 5-10 days [27].
• Kidney glomerular lesions were studied by light microscopy and immunofluorescence in 17 congenitally athymic Nude mice and 25 of their phenotypically normal littermates [16].
• Kidney tissue from five Nude mice was studied by electron microscopy [16].
• METHODS: Nude mice underwent subcutaneous injection with LS-174t colonic carcinoma cell line to generate carcinoma and were randomly separated into two groups [28].
• Two examples of animal thyroid tissue xenotransplantation onto nude mice are briefly discussed: Nude mice bearing normal thyroid tissue transplants from 4 different species (man, rat, pig, guinea-pig) have been used for the study of the species specific effect of bovine TSH and TSAb [29].

References