High impact information on CYP17A1

• Thus, the induction of b5 in the FZ and of P450c17 in the TZ of the fetal adrenal late in gestation coincided temporally with the prepartum rise in dehydroepiandrosterone previously reported [1].
• However, the lack of cytochrome b5 and P450c17 expression in the FZ of the developing macaque adrenal cortex for much of the second and third trimesters distinguishes it from the mature zona reticularis seen in adult animals [1].
• The relative abundance of P450c17 mRNA was increased in the fetal zone after metyrapone treatment [2].
• The protein-coding region of marmoset and rhesus P450c17 cDNAs were then isolated from adrenals using RT-PCR/TA cloning [3].

Anatomical context of CYP17A1

• Whereas P450c17 was induced largely in the transitional zone (TZ; outer-most FZ), b5 expression was strongest in FZ cells further from the capsule, although overlap between these regions involved a narrow band of cells beneath the TZ that may represent the developing zona reticularis [1].

Analytical, diagnostic and therapeutic context of CYP17A1

• The developmental expressions of cytochrome b5 (b5), 17 alpha-hydroxylase/17,20-lyase cytochrome P450 (P450c17), and 3 beta-hydroxysteroid dehydrogenase were examined in primate fetal adrenals by immunocytochemistry from 50-160 d gestation [1].

References