Disease relevance of ST

• We identified several ST in Arabidopsis resembling the C-terminus of the Arabidopsis two-domain ST1 and the single-domain GlpE protein from Escherichia coli [1].

High impact information on ST

• Based on sequence homology, the plant ST coding sequences are grouped under the SULT3 family, also known as the flavonol ST family [2].
• This protein is categorized as single-domain sulfurtransferase and is annotated as a senescence-associated protein (sen1-like protein) and ketoconazole resistance protein (http://arabidopsis.org/info/genefamily/STR_genefamily.html) [3].
• Incubation with 1-aminocyclopropane-1-carboxylic acid, the precursor of ethylene, increased while fumigation with ethylene decreased expression and activity of ST and CAS [4].
• However, the changes in RNA accumulation and protein levels of ST and CAS did not correlate with the expression of these senescence marker genes; the specific ST and CAS activities either decreased (ST) or increased (CAS) [4].
• All ST contain distinct structural domains, and can exist as single-domain proteins, as tandemly repeated modules in which the C-terminal domain bears the active site, or as members of multi-domain proteins [1].

Biological context of ST

• A database search for similarities between sequenced parts of the Arabidopsis thaliana genome with known sulfurtransferase sequences from Escherichia coli and mammals was undertaken to obtain information about plant sulfurtransferase-like proteins [5].
• The three desulfoglucosinolate sulfotransferase proteins in Arabidopsis have different substrate specificities and are differentially expressed [6].

Associations of ST with chemical compounds

• Crystal structure of At2g03760, a putative steroid sulfotransferase from Arabidopsis thaliana [7].
• Recently a sulfurtransferase specific for the substrate 3-mercaptopyruvate was isolated from Arabidopsis thaliana [Papenbrock, J. & Schmidt, A. (2000) Eur. J. Biochem. 267, 145-154] [8].

Other interactions of ST

• A cDNA clone (RaRO47) encoding a sulfotransferase (ST) has been isolated from Arabidopsis cell suspensions [9].
• The existence of rhodanese (thiosulfate:cyanide sulfurtransferase; EC 2.8.1.1) in plants has been highly controversial [10].

Analytical, diagnostic and therapeutic context of ST

• It was found that, at the level of steady-state mRNA, expression of gene(s) encoding this ST was rapidly induced in the aerial parts of young seedlings, and during growth of Arabidopsis cell cultures [9].
• Molecular cloning of a sulfotransferase in Arabidopsis thaliana and regulation during development and in response to infection with pathogenic bacteria [9].
• Results of a Southern blot performed with genomic Arabidopsis DNA showed the occurrence of at least two sulfurtransferase-like isozymes in Arabidopsis [5].

References