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Gene Review

CCR2  -  glycine-rich RNA-binding protein 7

Arabidopsis thaliana

Synonyms: "cold, circadian rhythm, and rna binding 2", ATGPR7, ATGRP7, F2G1.4, GLYCINE RICH PROTEIN 7, ...
 
 
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Disease relevance of ATGRP7

  • Heterologous expression of CSDP1 or GRP7 complemented the cold sensitivity of BX04 mutant Escherichia coli that lack four cold shock proteins (CSPs) and is highly sensitive to cold stress, and resulted in better survival rate than control cells during incubation at low temperature [1].
 

High impact information on ATGRP7

  • A transcript encoding a glycine-rich RNA-binding protein, Atgrp7, in Arabidopsis thaliana undergoes circadian oscillations with peak levels in the evening [2].
  • AtGRP7 also influences the choice of splice sites in the Atgrp8 transcript encoding a related RNA-binding protein, favoring the production of an alternatively spliced, unstable Atgrp8 transcript [3].
  • This conservation points to the importance of this regulatory mechanism to control the level of the clock-regulated glycine-rich RNA-binding proteins and shows how AtGRP7 can control abundance of target transcripts [3].
  • Recombinant AtGRP7 protein specifically interacts with the 3' untranslated region and the intron of its transcript, suggesting that the shift in splice site selection and downregulation involves binding of AtGRP7 to its pre-mRNA [3].
  • The circadian clock regulated RNA-binding protein AtGRP7 autoregulates its expression by influencing alternative splicing of its own pre-mRNA [3].
 

Biological context of ATGRP7

  • Arabidopsis transportin1 is the nuclear import receptor for the circadian clock-regulated RNA-binding protein AtGRP7 [4].
  • A tRNA(Leu)-like sequence is located within a probable enhancer region of the RNA polymerase II-dependent gene encoding an RNA-binding protein, Atgrp7, in Arabidopsis (Mol. Gen. Genet. 261 (1999) 811) [5].
  • A 265-bp fragment upstream of the transcription start site is necessary for high-amplitude Atgrp7 cycling [6].
  • Fusion of a 1.5-kb promoter fragment to the beta-glucuronidase (gus) reporter gene leads to circadian oscillations in the level of the gus transcript in transgenic Arabidopsis plants, with highest levels in the evening, indicating that transcription of the Atgrp7 gene is rhythmically activated by the endogenous circadian clock [6].
  • Selective evolution of ligand by exponential enrichment (SELEX) revealed that GRP7 does not recognize specific RNAs but binds preferentially to G-rich RNA sequences [1].
 

Anatomical context of ATGRP7

  • In addition, fluorescently labelled AtGRP7 was specifically imported into nuclei of permeabilized HeLa cells by Arabidopsis AtTRN1 and human TRN1 [4].
 

Associations of ATGRP7 with chemical compounds

 

Other interactions of ATGRP7

  • The proteins encoded by AtGRP7 and AtGRP8 may be members of a family of proteins which serve a vital role in RNA transcription or processing during stress [8].
  • Regulation of AtGRP7 downstream targets also depends on its RNA-binding activity, as AtGRP8 and other targets identified by transcript profiling of wild-type and AtGRP7-ox plants using fluorescent differential display are negatively affected by AtGRP7 but not by AtGRP7-RQ [9].
 

Analytical, diagnostic and therapeutic context of ATGRP7

  • Genetic crosses between a line bearing a promoter-gus fusion and plants that overexpress AtGRP7 show that the promoter by itself does not mediate the negative feedback of AtGRP7 on the oscillations of its own transcript [6].

References

  1. Cold shock domain proteins and glycine-rich RNA-binding proteins from Arabidopsis thaliana can promote the cold adaptation process in Escherichia coli. Kim, J.S., Park, S.J., Kwak, K.J., Kim, Y.O., Kim, J.Y., Song, J., Jang, B., Jung, C.H., Kang, H. Nucleic Acids Res. (2007) [Pubmed]
  2. AtGRP7, a nuclear RNA-binding protein as a component of a circadian-regulated negative feedback loop in Arabidopsis thaliana. Heintzen, C., Nater, M., Apel, K., Staiger, D. Proc. Natl. Acad. Sci. U.S.A. (1997) [Pubmed]
  3. The circadian clock regulated RNA-binding protein AtGRP7 autoregulates its expression by influencing alternative splicing of its own pre-mRNA. Staiger, D., Zecca, L., Wieczorek Kirk, D.A., Apel, K., Eckstein, L. Plant J. (2003) [Pubmed]
  4. Arabidopsis transportin1 is the nuclear import receptor for the circadian clock-regulated RNA-binding protein AtGRP7. Ziemienowicz, A., Haasen, D., Staiger, D., Merkle, T. Plant Mol. Biol. (2003) [Pubmed]
  5. A tRNA(Leu)-like sequence located immediately upstream of an Arabidopsis clock-regulated gene is transcriptionally active: efficient transcription by an RNA polymerase III-dependent in vitro transcription system. Hasegawa, K., Yukawa, Y., Obokata, J., Sugiura, M. Gene (2003) [Pubmed]
  6. Circadian clock-regulated expression of an RNA-binding protein in Arabidopsis: characterisation of a minimal promoter element. Staiger, D., Apel, K. Mol. Gen. Genet. (1999) [Pubmed]
  7. AtGRP7 is involved in the regulation of abscisic acid and stress responses in arabidopsis. Cao, S., Jiang, L., Song, S., Jing, R., Xu, G. Cell. Mol. Biol. Lett. (2006) [Pubmed]
  8. Two cDNAs from Arabidopsis thaliana encode putative RNA binding proteins containing glycine-rich domains. van Nocker, S., Vierstra, R.D. Plant Mol. Biol. (1993) [Pubmed]
  9. Auto-regulation of the circadian slave oscillator component AtGRP7 and regulation of its targets is impaired by a single RNA recognition motif point mutation. Schöning, J.C., Streitner, C., Page, D.R., Hennig, S., Uchida, K., Wolf, E., Furuya, M., Staiger, D. Plant J. (2007) [Pubmed]
 
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