The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

Links

 

Gene Review

CAX2  -  low affinity calcium antiporter CAX2

Arabidopsis thaliana

Synonyms: MDC11.19, atcax2, cation exchanger 2
 
 
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.
 

High impact information on CAX2

 

Biological context of CAX2

  • To determine whether CAX1 and CAX2 activity is affected by pH, Ca(2+)/H(+) antiport activity was measured in vacuolar membrane vesicles isolated from yeast heterologously expressing either transporter [4].
  • Here we demonstrate that unlike cax1 mutants, CAX2 insertional mutants caused no discernable morphological phenotypes or alterations in Ca2+/H+ antiport activity [5].
  • Heterologous expression in yeast identified an N-terminal regulatory region in CAX2, suggesting that Arabidopsis contains multiple cation/H+ antiporters with shared regulatory features [5].
  • Analysis of a CAX2 promoter beta-glucoronidase (GUS) reporter gene fusion confirmed that CAX2 was expressed throughout the plant and strongly expressed in flower tissue, vascular tissue and in the apical meristem of young plants [5].
  • Previously, we made a chimeric Arabidopsis thaliana vacuolar transporter CAX2B [a variant of N-terminus truncated form of CAX2 (sCAX2) containing the "B" domain from CAX1] that has enhanced calcium (Ca(2+)) substrate specificity and lost the manganese (Mn(2+)) transport capability of sCAX2 [6].
 

Associations of CAX2 with chemical compounds

  • Ca(2+) transport by CAX1 and CAX2 was regulated by cytosolic pH and each transporter had a distinct cytosolic pH profile [4].
  • Root Cd accumulation in high CAX4 and CAX2 expressing lines was increased in seedlings grown with 0.02 muM Cd [7].
  • Expression of an Arabidopsis CAX2 variant in potato tubers increases calcium levels with no accumulation of manganese [6].
 

Other interactions of CAX2

  • Experiments on vacuolar membrane-enriched vesicles isolated from yeast expressing CAX1 or CAX2 demonstrate that these genes encode high efficiency and low efficiency H+/Ca2+ exchangers, respectively [1].

References

  1. CAX1, an H+/Ca2+ antiporter from Arabidopsis. Hirschi, K.D., Zhen, R.G., Cunningham, K.W., Rea, P.A., Fink, G.R. Proc. Natl. Acad. Sci. U.S.A. (1996) [Pubmed]
  2. Manganese specificity determinants in the Arabidopsis metal/H+ antiporter CAX2. Shigaki, T., Pittman, J.K., Hirschi, K.D. J. Biol. Chem. (2003) [Pubmed]
  3. Structural determinants of Ca2+ transport in the Arabidopsis H+/Ca2+ antiporter CAX1. Shigaki, T., Cheng, N.H., Pittman, J.K., Hirschi, K. J. Biol. Chem. (2001) [Pubmed]
  4. Evidence of differential pH regulation of the Arabidopsis vacuolar Ca2+/H+ antiporters CAX1 and CAX2. Pittman, J.K., Shigaki, T., Hirschi, K.D. FEBS Lett. (2005) [Pubmed]
  5. Functional and regulatory analysis of the Arabidopsis thaliana CAX2 cation transporter. Pittman, J.K., Shigaki, T., Marshall, J.L., Morris, J.L., Cheng, N.H., Hirschi, K.D. Plant Mol. Biol. (2004) [Pubmed]
  6. Expression of an Arabidopsis CAX2 variant in potato tubers increases calcium levels with no accumulation of manganese. Kim, C.K., Han, J.S., Lee, H.S., Oh, J.Y., Shigaki, T., Park, S.H., Hirschi, K. Plant Cell Rep. (2006) [Pubmed]
  7. Enhanced Cd(2+)-selective root-tonoplast-transport in tobaccos expressing Arabidopsis cation exchangers. Koren'kov, V., Park, S., Cheng, N.H., Sreevidya, C., Lachmansingh, J., Morris, J., Hirschi, K., Wagner, G.J. Planta (2007) [Pubmed]
 
WikiGenes - Universities