Disease relevance of AT3G24050

• However, unlike the truncated tobacco GT-1a prepared from Escherichia coli extracts, the full-length Arabidopsis GT-1 bound to pea rbcS-3A box III and Arabidopsis chlorophyll a/b binding protein CAB2 light-responsive elements, both of which contain GATA motifs [1].

High impact information on AT3G24050

• The tobacco nuclear proteins CUF-1 (CAB upstream factor 1) and CGF-1 (CAB GATA factor 1) bind the Arabidopsis CAB2 promoter, and the CGF-1 binding site is contained within a minimal clock- and phytochrome-regulated region of the promoter [2].
• One of these lines, BME3, had a T-DNA insertion site in the 5' upstream region of a GATA-type zinc finger transcription factor gene (termed BME3-ZF) [3].
• Genetic analysis of Arabidopsis GATA transcription factor gene family reveals a nitrate-inducible member important for chlorophyll synthesis and glucose sensitivity [4].
• Our results support multiple models of evolution of the GATA gene family in plants including gene duplication and exon shuffling [5].
• Herein, we show that the Arabidopsis and the rice (Oryza sativa) genomes present 29 and 28 loci, respectively, that encode for putative GATA factors [5].

Biological context of AT3G24050

• Type-IV zinc-finger proteins have been extensively characterised in animals and fungi and are referred to as GATA factors by virtue of their affinity for promoter elements containing this sequence [6].
• Trans-activation of gene expression by the four Arabidopsis proteins via some, but not all, DNA elements tested indicates that the Arabidopsis proteins can form functional interactions with previously defined promoter elements containing GATA motifs [6].
• The phylogeny of the GATA zinc finger motif, excluding flanking regions, evidenced the existence of four classes of GATA zinc fingers, three of them containing 18 residues in the zinc finger loop and one containing a 20-residue loop [5].
• The presence of GATA-like motifs in the upstream region of the Acl1.4 gene (encoding for ACP4) and the similarity in light-mediated induction to ferredoxin-A mRNA suggests a direct role of light in Acl1.4 gene activation [7].
• Molecular evolution of the GATA family of transcription factors: conservation within the DNA-binding domain [8].

Associations of AT3G24050 with chemical compounds

• In plants, GATA DNA motifs have been implicated in light-dependent and nitrate-dependent control of transcription [5].
• The Dof domain, which is composed of 52 amino acid residues, is similar to the Cys2/Cys2 zinc finger DNA-binding domain of GATA1 and steroid hormone receptors, but has a longer putative loop than that in the case of these zinc finger domains [9].
• The Arabidopsis gene ZIM encodes a putative transcription factor containing a novel GATA-type zinc-finger domain with a longer spacer between its two sets of conserved cysteine residues (C-X2-C-X20-C-X2-C) [10].

Physical interactions of AT3G24050

• In addition, we showed that GATA-1 could also bind specifically to a cis-acting element in the promoter of the GAPA gene, which is coordinately regulated by light with the GAPB gene [11].

Analytical, diagnostic and therapeutic context of AT3G24050

• Gel mobility shift assays showed that GATA-1, but not GATA-20, binds specifically to the two GATA motifs within the XXIII fragment [11].

References