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Gene Review:

HSP18.2 - heat shock protein 18.2

Arabidopsis thaliana

Synonyms: MTH12.7, MTH12_7

Matsuhara, S. et al., Masclaux, F. et al., Takahashi, T. et al., Shinmyo, A. et al., Zhang, L. et al., et al.

Shinmyo, Shoji, Bando, Nagaya, Nakai, Kato, Sekine, Yoshida,

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Disease relevance of HSP18.2

The 5' region of the HSP18.2 gene has been fused, in frame, to the uidA gene from Escherichia coli which encodes beta-glucuronidase (GUS), and the product has been introduced into petunia by Agrobacterium-mediated transformation [1].

High impact information on HSP18.2

Our results suggest that Arabidopsis HSFs immunoreactive for HSF1 antibodies bind constitutively to the HSP18.2 gene [2].
We show that, irrespective of the location of the T-DNA insertion, an HSP18.2 promoter towards the left border of the T-DNA effectively induces transcription of flanking genome sequences in response to heat shock [3].
This paper describes expression profiles of the Arabidopsis HSP18.2 heat-shock gene promoter by using three different reporter genes, and the application of this promoter to a method we have developed to drive heat-shock-dependent transcription of plant genome DNA flanked by T-DNA insertions [3].
In this paper, we describe a gene expression system driven by a heat-shock gene promoter (HSP18.2), to trigger the expression of an intron-containing inverted-repeat [4].
To evaluate the efficiency of this system, RNA corresponding to the phytoene desaturase gene of Arabidopsis thaliana was used as a reporter gene in transgenic plants and a comparative study was performed using either the CaMV35S constitutive promoter or the HSP18.2 inducible promoter [4].

Biological context of HSP18.2

A 925 bp promoter fragment of a heat-shock protein gene (HSP18.2) of Arabidopsis thaliana showed clear heat-shock response of expression of the beta-glucuronidase (GUS) reporter gene in BY2 cells [5].
The molecular mechanisms underlying the differences between Hsp18.2 and Hsp70 in the kinetics of HSF1/promoter binding and corresponding mRNA expression profiles are discussed [6].

Anatomical context of HSP18.2

Transient expression of the HSP18.2 promoter in protoplasts was very low regardless of the heat shock [7].

Other interactions of HSP18.2

Using the technique of differential hybridization screening, we have isolated the cDNAs for two low-molecular-mass heat-shock proteins and their corresponding genes, HSP17.4 and HSP18.2, from Arabidopsis thaliana [1].
Suppressed phenylalanine ammonia-lyase activity after heat shock in transgenic Nicotiana plumbaginifolia containing an Arabidopsis HSP18.2-parsley PAL2 chimera gene [8].

References

Characterization of two genes encoding small heat-shock proteins in Arabidopsis thaliana. Takahashi, T., Komeda, Y. Mol. Gen. Genet. (1989) [Pubmed]
Analysis of heat-shock transcription factor-DNA binding in Arabidopsis suspension cultures by UV laser crosslinking. Zhang, L., Eggers-Schumacher, G., Schöffl, F., Prändl, R. Plant J. (2001) [Pubmed]
Heat-shock tagging: a simple method for expression and isolation of plant genome DNA flanked by T-DNA insertions. Matsuhara, S., Jingu, F., Takahashi, T., Komeda, Y. Plant J. (2000) [Pubmed]
Gene silencing using a heat-inducible RNAi system in Arabidopsis. Masclaux, F., Charpenteau, M., Takahashi, T., Pont-Lezica, R., Galaud, J.P. Biochem. Biophys. Res. Commun. (2004) [Pubmed]
Metabolic engineering of cultured tobacco cells. Shinmyo, A., Shoji, T., Bando, E., Nagaya, S., Nakai, Y., Kato, K., Sekine, M., Yoshida, K. Biotechnol. Bioeng. (1998) [Pubmed]
Heat stress-dependent DNA binding of Arabidopsis heat shock transcription factor HSF1 to heat shock gene promoters in Arabidopsis suspension culture cells in vivo. Zhang, L., Lohmann, C., Prändl, R., Schöffl, F. Biol. Chem. (2003) [Pubmed]
Heat-inducible expression system for a foreign gene in cultured tobacco cells using the HSP18.2 promoter of Arabidopsis thaliana. Yoshida, K., Kasai, T., Garcia, M.R., Sawada, S., Shoji, T., Shimizu, S., Yamazaki, K., Komeda, Y., Shinmyo, A. Appl. Microbiol. Biotechnol. (1995) [Pubmed]
Suppressed phenylalanine ammonia-lyase activity after heat shock in transgenic Nicotiana plumbaginifolia containing an Arabidopsis HSP18.2-parsley PAL2 chimera gene. Moriwaki, M., Yamakawa, T., Washino, T., Kodama, T., Igarashi, Y. J. Biosci. Bioeng. (1999) [Pubmed]

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