High impact information on HYL1

• A mutation in the Arabidopsis HYL1 gene encoding a dsRNA binding protein affects responses to abscisic acid, auxin, and cytokinin [1].
• We present evidence that the HYL1 gene is ABA-regulated and encodes a nuclear dsRNA binding protein [1].
• In the present work we investigated the role of the Arabidopsis nuclear RNA-binding protein HYL1 and the nuclear RNase III enzyme DCL1 in processing of primary miRNA (pri-miR171a) [2].
• A knockout mutation of the hyl1 gene is recessive and pleiotropic, causing developmental abnormalities, increasing sensitivity to abscisic acid, and reducing sensitivity to auxin and cytokinin [3].
• However, double-stranded RNA-induced posttranscriptional gene silencing is unaffected by the hyl1 mutation [3].

Biological context of HYL1

• The nuclear dsRNA binding protein HYL1 is required for microRNA accumulation and plant development, but not posttranscriptional transgene silencing [4].
• The interaction between DCL1 and HYL1 is important for efficient and precise processing of pri-miRNA in plant microRNA biogenesis [5].
• Because hyl1 mutants show marked abnormalities in hormone responses, these results further suggest that miRNA-mediated changes in mRNA stability play a vital role in plant hormone signaling [3].

Associations of HYL1 with chemical compounds

• These results indicate that each HYL1/DRB family protein interacts with one specific partner among the four Dicer-like proteins [6].

Physical interactions of HYL1

• An immunoprecipitation assay using Agrobacterium-mediated transient expression in Nicotiana benthamiana showed that HYL1 was able to form a complex with wild-type DCL1 protein, but not with the dcl1-9 mutant protein [5].

Other interactions of HYL1

• Genetic analysis supports that SE and HYL1 act in the same pathway to regulate plant development [7].

Analytical, diagnostic and therapeutic context of HYL1

• In situ hybridization indicates that HYL1 gene is preferentially expressed in leaf blades as well as vasculature [8].
• For genetic analysis of the mechanism of leaf curvature, we chose hyl1 mutant of Arabidopsis as a model for dissection of leaf venation pattern and adaxial/abaxial polarity [8].

References