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NRT1.1  -  nitrate transporter 1.1

Arabidopsis thaliana

Synonyms: ARABIDOPSIS THALIANA NITRATE TRANSPORTER 1, ATNRT1, ATNRT1.1, B-1, CHL1, ...
 
 
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Disease relevance of NRT1.1

  • This uptake deficiency was reversed when a CHL1 cDNA clone driven by the cauliflower mosaic virus 35S promoter was expressed in transgenic chl1 plants [1].
 

High impact information on NRT1.1

  • CHL1 mRNA is found predominantly in roots and displays nitrate- and pH-dependent regulation [2].
  • Injection of CHL1 mRNA into Xenopus oocytes produces a nitrate- and pH-dependent membrane depolarization, inward current, and nitrate uptake [2].
  • The CHL1 gene of Arabidopsis, which when mutated confers resistance to the herbicide chlorate and a decrease in nitrate uptake, was isolated and found to encode a protein with 12 putative membrane-spanning segments [2].
  • Thus, we suggest that it is the lack of NRT1.1 activity, rather than the absence of this transporter, that derepresses NRT2.1 expression in the presence of NH(4)(+) [3].
  • Arabidopsis thaliana mutants deficient for the NRT1.1 NO(3)(-) transporter display complex phenotypes, including lowered NO(3)(-) uptake, altered development of nascent organs, and reduced stomatal opening [3].
 

Biological context of NRT1.1

  • Consistent with this model, the NRT1.1 and ANR1 promoters both directed reporter gene expression in root primordia and root tips [4].
  • To obtain further insight at the molecular level on the multiple physiological functions of NRT1.1, we performed large-scale transcript profiling by serial analysis of gene expression in the roots of the chl1-5 deletion mutant of NRT1.1 and of the Columbia wild type [3].
  • Furthermore, transport studies on plants exposed to NO2- show that down-regulation of the NRT1.1 gene is associated with a decrease in NO3- influx [5].
  • Furthermore, although a control by external pH might contribute to the regulation of NRT1.1, changes in external pH due to lack of NR activity cannot alone explain the up-regulation of both genes [5].
  • The complexity of the molecular basis of nitrate uptake has been enhanced by the finding that in many plants both NRT1 and NRT2 classes are represented by multigene families [6].
 

Anatomical context of NRT1.1

 

Associations of NRT1.1 with chemical compounds

  • Here, we show that mutants of the NRT1.1 nitrate transporter display a strongly decreased root colonization of NO(3)(-)-rich patches, resulting from reduced lateral root elongation [4].
  • Sucrose supply prevents the inhibition of Nrt2;1 and Nrt1 expressions in the dark [8].
  • To test this, auxin regulation of CHL1 was examined [9].
 

Other interactions of NRT1.1

  • It is thus hypothesized that NRT1.1-mediated regulation of NRT2.1/NRT3.1 is a mechanism aiming to satisfy a specific NO(3)(-) demand of the plant in relation to the various specific roles that NO(3)(-) plays, in addition to being a N source [10].
  • Accordingly, nitrite (NO2-) strongly represses NRT1.1 and NIA1 transcript accumulation in the roots [5].
 

Analytical, diagnostic and therapeutic context of NRT1.1

  • In this study, we examined the in vivo function of CHL1 with in vivo uptake measurements and in situ hybridization experiments [1].

References

  1. CHL1 encodes a component of the low-affinity nitrate uptake system in Arabidopsis and shows cell type-specific expression in roots. Huang, N.C., Chiang, C.S., Crawford, N.M., Tsay, Y.F. Plant Cell (1996) [Pubmed]
  2. The herbicide sensitivity gene CHL1 of Arabidopsis encodes a nitrate-inducible nitrate transporter. Tsay, Y.F., Schroeder, J.I., Feldmann, K.A., Crawford, N.M. Cell (1993) [Pubmed]
  3. Transcript profiling in the chl1-5 mutant of Arabidopsis reveals a role of the nitrate transporter NRT1.1 in the regulation of another nitrate transporter, NRT2.1. Muños, S., Cazettes, C., Fizames, C., Gaymard, F., Tillard, P., Lepetit, M., Lejay, L., Gojon, A. Plant Cell (2004) [Pubmed]
  4. The Arabidopsis NRT1.1 transporter participates in the signaling pathway triggering root colonization of nitrate-rich patches. Remans, T., Nacry, P., Pervent, M., Filleur, S., Diatloff, E., Mounier, E., Tillard, P., Forde, B.G., Gojon, A. Proc. Natl. Acad. Sci. U.S.A. (2006) [Pubmed]
  5. Gene expression of the NO3- transporter NRT1.1 and the nitrate reductase NIA1 is repressed in Arabidopsis roots by NO2-, the product of NO3- reduction. Loqué, D., Tillard, P., Gojon, A., Lepetit, M. Plant Physiol. (2003) [Pubmed]
  6. Nitrate transport in plants: which gene and which control? Orsel, M., Filleur, S., Fraisier, V., Daniel-Vedele, F. J. Exp. Bot. (2002) [Pubmed]
  7. Eukaryotic nitrate and nitrite transporters. Galvan, A., Fernández, E. Cell. Mol. Life Sci. (2001) [Pubmed]
  8. Molecular and functional regulation of two NO3- uptake systems by N- and C-status of Arabidopsis plants. Lejay, L., Tillard, P., Lepetit, M., Olive, F., Filleur, S., Daniel-Vedele, F., Gojon, A. Plant J. (1999) [Pubmed]
  9. The Arabidopsis dual-affinity nitrate transporter gene AtNRT1.1 (CHL1) is regulated by auxin in both shoots and roots. Guo, F.Q., Wang, R., Crawford, N.M. J. Exp. Bot. (2002) [Pubmed]
  10. Regulation of the High-Affinity NO3- Uptake System by NRT1.1-Mediated NO3- Demand Signaling in Arabidopsis. Krouk, G., Tillard, P., Gojon, A. Plant Physiol. (2006) [Pubmed]
 
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