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APS2  -  ATP sulfurylase 2

Arabidopsis thaliana

Synonyms: ASA1, ATP SULFURYLASE ARABIDOPSIS 1, ATP SULFURYLASE PRECURSOR, F6F9.2, F6F9_2
 
 
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High impact information on APS2

  • Yeast artificial chromosome hybridization studies indicated that the PAI2 gene is tightly linked to the anthranilate synthase alpha subunit 1 (ASA1) gene on chromosome 5 [1].
  • Unlike previously known root ethylene-insensitive mutants, wei2 and wei3 were not affected in their response to auxin and showed a normal response to gravity [2].
  • Genetic mapping studies indicate that wei2 and wei3 correspond to previously unidentified ethylene pathway genes that may control cell-elongation processes functioning at the intersection of the ethylene and auxin response pathways [2].
  • No activity was detected for the second gene coding for a small subunit isoform (APS2) annotated in the Arabidopsis genome [3].
  • Expression of the three amt genes was higher in nitrogen-starved cells than in cells incubated in the presence of a source of nitrogen (either ammonium or nitrate), but amt1 was expressed at higher levels than the other two amt genes [4].
 

Biological context of APS2

 

Associations of APS2 with chemical compounds

  • As a consequence of altered AS kinetic properties, the trp5 mutants accumulated 3-fold higher soluble tryptophan than wild-type plants [6].
  • Compared with the enzyme from wild-type plants, the tryptophan concentration causing 50% inhibition of AS activity in the trp5 mutant increased nearly 3-fold, the apparent Km for chorismate decreased by approximately 50%, and the apparent Vmax increased 60% [6].
  • Three independent trp5 mutants defective in the Arabidopsis thaliana AS alpha subunit structural gene ASA1 were identified by selection for resistance to the herbicidal compound 6-methylanthranilate [6].
 

Analytical, diagnostic and therapeutic context of APS2

  • PCR techniques were used to isolate a full-length clone of a B. napus homologue of AMT1 from shoot mRNA which showed 97% similarity to AtAMT1;3 [7].

References

  1. Arabidopsis phosphoribosylanthranilate isomerase: molecular genetic analysis of triplicate tryptophan pathway genes. Li, J., Zhao, J., Rose, A.B., Schmidt, R., Last, R.L. Plant Cell (1995) [Pubmed]
  2. Five components of the ethylene-response pathway identified in a screen for weak ethylene-insensitive mutants in Arabidopsis. Alonso, J.M., Stepanova, A.N., Solano, R., Wisman, E., Ferrari, S., Ausubel, F.M., Ecker, J.R. Proc. Natl. Acad. Sci. U.S.A. (2003) [Pubmed]
  3. The different large subunit isoforms of Arabidopsis thaliana ADP-glucose pyrophosphorylase confer distinct kinetic and regulatory properties to the heterotetrameric enzyme. Crevillén, P., Ballicora, M.A., Mérida, A., Preiss, J., Romero, J.M. J. Biol. Chem. (2003) [Pubmed]
  4. Ammonium/methylammonium permeases of a Cyanobacterium. Identification and analysis of three nitrogen-regulated amt genes in synechocystis sp. PCC 6803. Montesinos, M.L., Muro-Pastor, A.M., Herrero, A., Flores, E. J. Biol. Chem. (1998) [Pubmed]
  5. Functional expression of Arabidopsis thaliana anthranilate synthase subunit I in Escherichia coli. Bernasconi, P., Walters, E.W., Woodworth, A.R., Siehl, D.L., Stone, T.E., Subramanian, M.V. Plant Physiol. (1994) [Pubmed]
  6. The Arabidopsis thaliana trp5 mutant has a feedback-resistant anthranilate synthase and elevated soluble tryptophan. Li, J., Last, R.L. Plant Physiol. (1996) [Pubmed]
  7. Regulation of the high-affinity ammonium transporter (BnAMT1;2) in the leaves of Brassica napus by nitrogen status. Pearson, J.N., Finnemann, J., Schjoerring, J.K. Plant Mol. Biol. (2002) [Pubmed]
 
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