High impact information on RHL1

• Our molecular and genetic data with double mutants, together with the expression analysis of a GL2 promoter-GUS reporter gene construct, indicate that the RHL1 gene acts independently of GL2 [1].
• The predicted RHL1 gene product is a small hydrophilic protein (38.9 kD) containing putative nuclear localization signals and shows no significant homology to any known amino acid sequence [1].
• Here we present a molecular analysis of the RHL1 (ROOT HAIRLESS 1) gene that, if mutated, prevents the formation of hairs on primary roots and causes a seedling lethal phenotype [1].
• RHL1 is an essential component of the plant DNA topoisomerase VI complex and is required for ploidy-dependent cell growth [2].
• HYP7 encodes the RHL1 (ROOT HAIRLESS 1) protein, and sequence analysis reveals that RHL1 has similarity to the C-terminal domain of mammalian DNA topo IIalpha, another type II topo that shares little sequence homology with topo VI [2].

Associations of RHL1 with chemical compounds

• On the other hand, roots treated with the ethylene inhibitor aminoethoxyvinyl-glycine, as well as roots of mutants affected in root hair initiation (rhl1, rhd6-1, and axr2-1) revealed no localized increases of XET action at all and consequently did not initiate root hairs [3].

Other interactions of RHL1

• Mutant phenotypes in the patterning and shape of leaf trichomes in rhl1, rhl2, rhl3 and erh3 were particularly obvious [4].
• We have examined the genetic relationship between RHL1 and GL2, an inhibitor of root hair initiation in non-hair cells [1].

References