Disease relevance of TUA1

• Chickens were immunized with the lipid emulsion system and Salmonella typhimurium mitogen adjuvant for fowl plus synthetic peptides corresponding to the divergent amino acid sequences at the carboxyl terminus of each of two different alpha-tubulin isoforms from Arabidopsis thaliana [1].

High impact information on TUA1

• Here, Arabidopsis hypocotyl microtubules wershee labelled with AtEB1a-GFP (Arabidopsis microtubule end-binding protein 1a) or GFP-TUA6 (Arabidopsis alpha-tubulin 6) to record long cycles of reorientation [2].
• TUA1 and TUA3 were characterized earlier in our laboratory [3].
• Indirect immunofluorescence of alpha-tubulin revealed that very few microtubules were present in mutant cells, resulting in the total lack of the parallel microtubule organization that is typical of elongating cells in the wild type [4].
• Multimode fluorescence resonance energy transfer (FRET) microscopy reveals a direct physical interaction of AtTFCB with alpha tubulin in living plant cells [5].
• Transgenic tobacco plants carrying the diphtheria toxin chain A gene under the control of the alpha 1-tub promoter were of normal phenotype but seed fertility was drastically reduced [6].

Biological context of TUA1

• Furthermore, the transgene could not be transmitted to the next generation through pollen, supporting the observation that the alpha 1-tub promoter is active only in pollen [6].
• Pollen-specific expression of the Arabidopsis thaliana alpha 1-tubulin promoter assayed by beta-glucuronidase, chloramphenicol acetyltransferase and diphtheria toxin reporter genes [6].

Other interactions of TUA1

• The expression levels of all six alpha-tubulin genes, TUA1-TUA6, were increased by increasing gravity, although the extent was variable among genes [7].

References