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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Gene Review: 

RPO41  -  Rpo41p

Saccharomyces cerevisiae S288c

Synonyms: DNA-directed RNA polymerase, mitochondrial, YFL036W
 
 
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Disease relevance of RPO41

  • Rpo41 is similar to the single polypeptide bacteriophage T7RNAP, which does not require additional factors for promoter-selective initiation but whose activity is modulated during infection by association with T7 lysozyme [1].
 

High impact information on RPO41

  • We have used two-hybrid and fusion protein constructs to analyze the requirements for interaction between the single subunit core polymerase (Rpo41p), and the sigma-like promoter specificity factor (Mtf1p) [2].
  • Yeast RPO41 gene product is required for transcription and maintenance of the mitochondrial genome [3].
  • The RPO41 gene is located on chromosome VI, as determined by hybridization to electrophoretically separated yeast chromosomes [3].
  • Two forms of RPO41-dependent RNA polymerase. Regulation of the RNA polymerase by glucose repression may control yeast mitochondrial gene expression [4].
  • The alternative mtDNA replication mechanism provided by ori/rep is independent of mitochondrial RNA polymerase encoded by RPO41 as a HS rho(-) genome is stably maintained in a mgm101, rpo41 double mutant [5].
 

Biological context of RPO41

  • A new point mutation in the nuclear gene of yeast mitochondrial RNA polymerase, RPO41, identifies a functionally important amino-acid residue in a protein region conserved among mitochondrial core enzymes [6].
  • We identified Rpo41 mutant E1224A as having reduced interactions with Mtf1 in a two-hybrid assay and a temperature-sensitive petite phenotype in vivo [1].
  • The yeast mitochondrial RNA polymerase (RNAP) is a two-subunit enzyme composed of a catalytic core (Rpo41) and a specificity factor (Mtf1) encoded by nuclear genes [7].
 

Anatomical context of RPO41

  • The results indicate that suppression is mediated by an increased level of MTF1 protein in mitochondria of the rpo41/pet-ts798 mutant [8].
 

Other interactions of RPO41

  • These genes do not appear to regulate mitochondrial transcript levels via regulation of the nuclear genes RPO41 and MTF1, which encode the subunits of the mitochondrial RNA polymerase [9].

References

  1. A mutation in the yeast mitochondrial core RNA polymerase, Rpo41, confers defects in both specificity factor interaction and promoter utilization. Matsunaga, M., Jaehning, J.A. J. Biol. Chem. (2004) [Pubmed]
  2. Identification of three regions essential for interaction between a sigma-like factor and core RNA polymerase. Cliften, P.F., Park, J.Y., Davis, B.P., Jang, S.H., Jaehning, J.A. Genes Dev. (1997) [Pubmed]
  3. Yeast RPO41 gene product is required for transcription and maintenance of the mitochondrial genome. Greenleaf, A.L., Kelly, J.L., Lehman, I.R. Proc. Natl. Acad. Sci. U.S.A. (1986) [Pubmed]
  4. Two forms of RPO41-dependent RNA polymerase. Regulation of the RNA polymerase by glucose repression may control yeast mitochondrial gene expression. Wilcoxen, S.E., Peterson, C.R., Winkley, C.S., Keller, M.J., Jaehning, J.A. J. Biol. Chem. (1988) [Pubmed]
  5. The mitochondrial nucleoid protein, Mgm101p, of Saccharomyces cerevisiae is involved in the maintenance of rho(+) and ori/rep-devoid petite genomes but is not required for hypersuppressive rho(-) mtDNA. Zuo, X.M., Clark-Walker, G.D., Chen, X.J. Genetics (2002) [Pubmed]
  6. A new point mutation in the nuclear gene of yeast mitochondrial RNA polymerase, RPO41, identifies a functionally important amino-acid residue in a protein region conserved among mitochondrial core enzymes. Lisowsky, T., Stein, T., Michaelis, G., Guan, M.X., Chen, X.J., Clark-Walker, G.D. Curr. Genet. (1996) [Pubmed]
  7. Mutations in the yeast mitochondrial RNA polymerase specificity factor, Mtf1, verify an essential role in promoter utilization. Karlok, M.A., Jang, S.H., Jaehning, J.A. J. Biol. Chem. (2002) [Pubmed]
  8. A point mutation in the core subunit gene of yeast mitochondrial RNA polymerase is suppressed by a high level of specificity factor MTF1. Riemen, G., Michaelis, G. Mol. Gen. Genet. (1993) [Pubmed]
  9. Glucose repression of yeast mitochondrial transcription: kinetics of derepression and role of nuclear genes. Ulery, T.L., Jang, S.H., Jaehning, J.A. Mol. Cell. Biol. (1994) [Pubmed]
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