High impact information on CHA4

• This regulation requires Cha4, a serine-dependent activator that binds to the SRG1 promoter and is required for SRG1 induction in the presence of serine [1].
• In addition, deletion of the gene-specific activator Cha4p did not affect derepression of CHA1 in cells depleted for Swh3p [2].
• We have previously shown that the transcriptional activator protein Cha4p mediates serine/threonine induction of CHA1 expression [3].
• We also performed "domain swaps" between Leu3p and Cha4p, a serine/threonine-responsive activator that, like Leu3p, belongs to the family of Zn(II)2Cys6 proteins [4].
• Molecular and genetic mapping positioned the cha4 locus 17 cM centromere proximal to put1 on chromosome XII [5].

Biological context of CHA4

• Cha4p of Saccharomyces cerevisiae activates transcription via serine/threonine response elements [5].
• The cha4-1 allele causes noninducibility of a CHA1 p-lacZ translational gene fusion, indicating that Cha4p exerts its action through the CHA1 promoter [5].

Regulatory relationships of CHA4

• Thus, the H3 amino terminus is required to prevent Cha4p from activating CHA1 in the absence of inducer [6].

Other interactions of CHA4

• We conclude that Cha4p is a positive regulator of CHA1 transcription and that Cha4p alone, or as part of a complex, is binding UASCHA [5].

Analytical, diagnostic and therapeutic context of CHA4

• Furthermore, derepression in the absence of the H3 amino terminus requires the primary activator of this promoter, Cha4p, which we show by chromatin immunoprecipitation to be constitutively bound to the CHA1 promoter in WT yeast [6].

References