High impact information on MAS1

• Two DNA sequences that reduce mitotic fidelity of chromosome transmission have been identified: MIF1 and MIF2 [1].
• MIF1 is not essential for cell division but is necessary for the normal fidelity of chromosome transmission [1].
• Import of proteins into yeast mitochondria: the purified matrix processing protease contains two subunits which are encoded by the nuclear MAS1 and MAS2 genes [2].
• Import of proteins into yeast mitochondria: the nuclear MAS2 gene encodes a component of the processing protease that is homologous to the MAS1-encoded subunit [3].
• MAS1, a gene essential for yeast mitochondrial assembly, encodes a subunit of the mitochondrial processing protease [4].

Biological context of MAS1

• We have now cloned and sequenced the wild-type MAS1 gene and found that it encodes a subunit of the mitochondrial processing protease, that it is essential for cell viability and that the protein product participates in its own cleavage during import into mitochondria [4].
• The amino-terminal sequences of the two mature subunits were determined; comparison with the deduced amino acid sequences of the corresponding precursors revealed that the MAS1 and MAS2 subunits are synthesized with prepeptides composed of 19 and 13 residues, respectively, which have similar sequences [5].
• However MIF1 appears to be capable of interacting with the co-repressor TIF1beta and exhibits transcription repressor activity when fused to yeast GAL4 binding domain protein [6].

Anatomical context of MAS1

• Western blot analysis identified MIF1-like proteins in the murine trigeminal ganglion (TG) and immunostaining localized these proteins primarily to the cytoplasm of TG neuronal cell bodies [6].

Other interactions of MAS1

• This assumption was also tested by overexpressing the SMF1 gene in the temperature-sensitive mutant of the mitochondrial processing peptidase (MAS1) [7].

Analytical, diagnostic and therapeutic context of MAS1

• In situ hybridization for Mif1 transcripts confirms the selective expression of Mif1 in TG neurons [6].

References