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Gene Review:

CCW14 - Ccw14p

Saccharomyces cerevisiae S288c

Synonyms: Covalently-linked cell wall protein 14, ICWP, Inner cell wall protein, SSR1, YLR390W-A, ...

Moukadiri, I. et al., Andrés, I. et al., Mrsa, V. et al.

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High impact information on CCW14

For Ccw13p (encoded by DAN1 [also called TIR3]) and Ccw12p the association with the cell wall has not been described before; Ccw14p is identical with cell wall protein Icwp (I. Moukadiri, J. Armero, A. Abad, R. Sentandreu, and J. Zueco, J. Bacteriol. 179:2154-2162, 1997) [1].
Immunofluorescence analysis with the MAb showed that Icwp lies at the inner layer of the cell wall, being accessible to the antibody only after the outer layer of mannoproteins is disturbed by treatment with tunicamycin [2].
The screening of a lambda gt11 expression library enabled us to identify the open reading frame (ORF) coding for Icwp [2].
Both disruption and overexpression of the ORF caused increased sensitivities to calcofluor white and Congo red, while the disruption caused an increased sensitivity to Zymolyase digestion, suggesting for Icwp a structural role in association with glucan [2].
ICWP (EMBL accession number YLR391w, frame +3) codes for 238 amino acids, of which over 40% are serine or threonine, and contains a putative N-glycosylation site and a putative glycosylphosphatidylinositol attachment signal [2].

Analytical, diagnostic and therapeutic context of CCW14

The surface display of the protein A-Icwp fusion protein was positively monitored; however, direct immobilization of the cells on an IgG-Sepharose matrix did not produce the expected results, probably due to the fusion protein not being completely exposed on the surface of the cells [3].

References

Deletion of new covalently linked cell wall glycoproteins alters the electrophoretic mobility of phosphorylated wall components of Saccharomyces cerevisiae. Mrsa, V., Ecker, M., Strahl-Bolsinger, S., Nimtz, M., Lehle, L., Tanner, W. J. Bacteriol. (1999) [Pubmed]
Identification of a mannoprotein present in the inner layer of the cell wall of Saccharomyces cerevisiae. Moukadiri, I., Armero, J., Abad, A., Sentandreu, R., Zueco, J. J. Bacteriol. (1997) [Pubmed]
Immobilization of Saccharomyces cerevisiae cells to protein G-Sepharose by cell wall engineering. Andrés, I., Zueco, J., Parascandola, P. J. Mol. Microbiol. Biotechnol. (2003) [Pubmed]

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