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Gene Review

BDF1  -  Bdf1p

Saccharomyces cerevisiae S288c

Synonyms: Bromodomain-containing factor 1, L8084.18, YLR399C
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High impact information on BDF1

  • Efficient deposition of H2A.Z is further promoted by a specific pattern of histone H3 and H4 tail acetylation and the bromodomain protein Bdf1, a component of the Swr1 remodeling complex that deposits H2A.Z [1].
  • Our data also indicate that the in vivo binding of the transcription factor Bdf1 is associated with acetylation on most lysines but relative deacetylation on H4 lysine 16 [2].
  • Deletion of Bdf1 or the Taf67 Bdf-interacting domain leads to defects in gene expression [3].
  • The structural and functional similarities suggest that Bdf1 corresponds to the carboxy-terminal region of higher eukaryotic TAF(II)250 and that the interaction between TFIID and Bdf1 is important for proper gene expression [3].
  • Bromodomain point mutations that block Bdf1 binding to histones disrupt transcription and reduce Bdf1 association with chromatin in vivo [4].

Biological context of BDF1

  • We have selected multicopy extragenic suppressor genes, revealing that this phenotype can be suppressed by overdosing the transcription factors BDF1 and GAT1 in the yaf9Delta strain [5].
  • Furthermore, yeast strain lacking the BDF1 gene shows the Spt phenotype that is shown also by the ASF1 gene disruptant, and a double-knockout strain of both genes shows synthetic lethality, indicating that ASF1 genetically interacts with bromodomains associated with yTFIID [6].
  • In this report, genetic analysis indicated that the salt sensitivity of the BDF1 deletion mutant was suppressed by increased gene dosage of its homologous gene BDF2 [7].
  • Surprisingly, deletion of BDF1 or a Bdf1 mutation that abolishes histone binding leads to transcriptional downregulation of genes located at heterochromatin-euchromatin boundaries [8].
  • Representatives of the TFIID pathway include the TATA binding protein, TAF1, and Bdf1 [9].

Anatomical context of BDF1

  • The data derived from assays for polyuridine-directed polyphenylalanine synthesis of isolated ribosomes demonstrated that the ID50 values obtained were consistent with the observed inhibition of whole cell protein synthesis inhibition for P-388 cells, as well as for BDF1 and DBA/2 liver cells [10].

Other interactions of BDF1

  • The bromodomain-containing protein Bdf1p acts as a phenotypic and transcriptional multicopy suppressor of YAF9 deletion in yeast [5].
  • Moreover, like Htz1 and Bdf1, the SWR-C promotes gene expression near silent heterochromatin [11].
  • The findings suggest that Bdf1 blocks assembly, whereas Mot1 promotes disassembly of the transcription machinery [9].
  • Biochemical experiments indicate that Bdf1 competes with the Sir2 deacetylase for binding to acetylated histone H4 [8].

Analytical, diagnostic and therapeutic context of BDF1

  • Using a yeast two-hybrid assay we detected an interaction between the N-terminal region of histone H4 (amino acids 1--59) and a fragment of the bromodomain factor 1 protein (Bdf1p) (amino acids 304--571) that includes one of the two bromodomains of this protein [12].


  1. Histone variant H2A.Z marks the 5' ends of both active and inactive genes in euchromatin. Raisner, R.M., Hartley, P.D., Meneghini, M.D., Bao, M.Z., Liu, C.L., Schreiber, S.L., Rando, O.J., Madhani, H.D. Cell (2005) [Pubmed]
  2. Mapping global histone acetylation patterns to gene expression. Kurdistani, S.K., Tavazoie, S., Grunstein, M. Cell (2004) [Pubmed]
  3. Bromodomain factor 1 corresponds to a missing piece of yeast TFIID. Matangkasombut, O., Buratowski, R.M., Swilling, N.W., Buratowski, S. Genes Dev. (2000) [Pubmed]
  4. Different sensitivities of bromodomain factors 1 and 2 to histone H4 acetylation. Matangkasombut, O., Buratowski, S. Mol. Cell (2003) [Pubmed]
  5. The bromodomain-containing protein Bdf1p acts as a phenotypic and transcriptional multicopy suppressor of YAF9 deletion in yeast. Bianchi, M.M., Costanzo, G., Chelstowska, A., Grabowska, D., Mazzoni, C., Piccinni, E., Cavalli, A., Ciceroni, F., Rytka, J., Slonimski, P.P., Frontali, L., Negri, R. Mol. Microbiol. (2004) [Pubmed]
  6. Identification and characterization of CIA/ASF1 as an interactor of bromodomains associated with TFIID. Chimura, T., Kuzuhara, T., Horikoshi, M. Proc. Natl. Acad. Sci. U.S.A. (2002) [Pubmed]
  7. Genetic and Comparative Transcriptome Analysis of Bromodomain Factor 1 in the Salt Stress Response of Saccharomyces cerevisiae. Liu, X., Zhang, X., Wang, C., Liu, L., Lei, M., Bao, X. Curr. Microbiol. (2007) [Pubmed]
  8. Bromodomains mediate an acetyl-histone encoded antisilencing function at heterochromatin boundaries. Ladurner, A.G., Inouye, C., Jain, R., Tjian, R. Mol. Cell (2003) [Pubmed]
  9. Changes in genomewide occupancy of core transcriptional regulators during heat stress. Zanton, S.J., Pugh, B.F. Proc. Natl. Acad. Sci. U.S.A. (2004) [Pubmed]
  10. Antitumor agents LIX: effects of quassinoids on protein synthesis of a number of murine tumors and normal cells. Hall, I.H., Liou, Y.F., Lee, K.H., Chaney, S.G., Willingham, W. Journal of pharmaceutical sciences. (1983) [Pubmed]
  11. A Snf2 family ATPase complex required for recruitment of the histone H2A variant Htz1. Krogan, N.J., Keogh, M.C., Datta, N., Sawa, C., Ryan, O.W., Ding, H., Haw, R.A., Pootoolal, J., Tong, A., Canadien, V., Richards, D.P., Wu, X., Emili, A., Hughes, T.R., Buratowski, S., Greenblatt, J.F. Mol. Cell (2003) [Pubmed]
  12. Bromodomain factor 1 (Bdf1) protein interacts with histones. Pamblanco, M., Poveda, A., Sendra, R., Rodríguez-Navarro, S., Pérez-Ortín, J.E., Tordera, V. FEBS Lett. (2001) [Pubmed]
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