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ENT1  -  Ent1p

Saccharomyces cerevisiae S288c

Synonyms: Epsin-1, YDL161W
 
 
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High impact information on ENT1

  • Here we describe two epsin amino-terminal homology (ENTH) domain-containing proteins, Ent3p and Ent5p, that are intimately involved in clathrin function at the Golgi [1].
  • On the basis of our results and the established roles of epsin and epsin-related proteins in endocytosis, we propose that ENTH-domain-containing proteins are a universal component of CCV formation [1].
  • Epsin UIMs are important for the internalization of receptors into vesicles at the plasma membrane [2].
  • In this study, we have characterized two homologous yeast proteins, Ent1p and Ent2p, which are similar to mammalian epsin [3].
  • The Ent1 protein was localized to peripheral and internal punctate structures, and biochemical fractionation studies found the protein associated with a large, Triton X-100-insoluble pellet [3].
 

Biological context of ENT1

  • The mammalian protein epsin is required for endocytosis [3].
  • Synthetic growth defects were observed in a pan1-20 ENT1(EE) double mutant, suggesting that Ent1p phosphorylation negatively regulates the formation/activity of a Pan1p-Ent1p complex [4].
  • We found that actin-regulating Ser/Thr kinase (ARK) mutants exhibit endocytic defects and that overexpressing either wild-type or alanine-substituted Ent1p partially suppressed phenotypes associated with loss of ARK kinases, including growth, endocytosis, and actin localization defects [4].
  • The goal of the present study was to analyse the haplotype structure and functionally characterize the variants of ENT1 [5].
  • Functional analysis in Saccharomyces cerevisiae revealed no differences in the kinetics of uptake of nucleosides and nucleoside analogs by the two non-synonymous variant transporters, ENT1-I216T and ENT1-E391K, and the reference ENT1 [5].
 

Anatomical context of ENT1

  • The yeast Epsin Ent1 is recruited to membranes through multiple independent interactions [6].
  • A physically interacting network of three classes of adaptors participate in clathrin-mediated traffic between the trans-Golgi network (TGN) and endosomes: AP-1, Gga proteins, and epsin-like proteins [7].
 

Associations of ENT1 with chemical compounds

  • Consistent with synthetic growth defects of pan1-20 ENT1(EE) cells, overexpressing glutamate-substituted Ent1p was deleterious to ARK mutants [4].
  • Converting both threonines to either glutamate or alanine mimics constitutively phosphorylated or dephosphorylated Ent1p, respectively [4].
  • The Ent1p bound PI(4,5)P2 and PI(3,5)P2 immobilized onto nitrocellulose in vitro and also weakly bound PI(3,4)P2, PI(3,4,5)P3, PI4P and PI5P [8].
 

Regulatory relationships of ENT1

 

Other interactions of ENT1

  • Yeast has two redundant genes encoding epsins, ENT1 and ENT2; deleting both genes is lethal [9].
  • In this study, we characterized the interaction of Ub with the Ede1 UBA domain and with the UIMs of Ent1 [6].
  • Epsin N-terminal homology domains perform an essential function regulating Cdc42 through binding Cdc42 GTPase-activating proteins [9].

References

  1. Yeast epsin-related proteins required for Golgi-endosome traffic define a gamma-adaptin ear-binding motif. Duncan, M.C., Costaguta, G., Payne, G.S. Nat. Cell Biol. (2003) [Pubmed]
  2. Epsins and Vps27p/Hrs contain ubiquitin-binding domains that function in receptor endocytosis. Shih, S.C., Katzmann, D.J., Schnell, J.D., Sutanto, M., Emr, S.D., Hicke, L. Nat. Cell Biol. (2002) [Pubmed]
  3. Yeast epsins contain an essential N-terminal ENTH domain, bind clathrin and are required for endocytosis. Wendland, B., Steece, K.E., Emr, S.D. EMBO J. (1999) [Pubmed]
  4. In vivo role for actin-regulating kinases in endocytosis and yeast epsin phosphorylation. Watson, H.A., Cope, M.J., Groen, A.C., Drubin, D.G., Wendland, B. Mol. Biol. Cell (2001) [Pubmed]
  5. Functional characterization in yeast of genetic variants in the human equilibrative nucleoside transporter, ENT1. Osato, D.H., Huang, C.C., Kawamoto, M., Johns, S.J., Stryke, D., Wang, J., Ferrin, T.E., Herskowitz, I., Giacomini, K.M. Pharmacogenetics (2003) [Pubmed]
  6. The yeast Epsin Ent1 is recruited to membranes through multiple independent interactions. Aguilar, R.C., Watson, H.A., Wendland, B. J. Biol. Chem. (2003) [Pubmed]
  7. Distinct roles for TGN/endosome epsin-like adaptors Ent3p and Ent5p. Costaguta, G., Duncan, M.C., Fernández, G.E., Huang, G.H., Payne, G.S. Mol. Biol. Cell (2006) [Pubmed]
  8. Fission yeast epsin, Ent1p is required for endocytosis and involved in actin organization. Sakamoto, C., Kawamoto, C., Takeuchi, K., Miyamoto, I., Shuntoh, H. The Kobe journal of medical sciences. (2004) [Pubmed]
  9. Epsin N-terminal homology domains perform an essential function regulating Cdc42 through binding Cdc42 GTPase-activating proteins. Aguilar, R.C., Longhi, S.A., Shaw, J.D., Yeh, L.Y., Kim, S., Schön, A., Freire, E., Hsu, A., McCormick, W.K., Watson, H.A., Wendland, B. Proc. Natl. Acad. Sci. U.S.A. (2006) [Pubmed]
 
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