High impact information on PRP11

• The yeast rna mutations (rna2-rna11) are a set of temperature-sensitive mutations that result in the accumulation of intron-containing mRNA precursors at the restrictive temperature [1].
• This strongly suggests that the PRP9-SPP91-PRP11 complex is a key element of the splicing machinery [2].
• Here it is demonstrated that protein-protein interactions occur between SPP91 and PRP11 [2].
• The combination of the prp9-1 mutant and a truncated prp11 mutant exhibits a synthetic lethal phenotype, suggestive of a common biochemical defect [2].
• The yeast MUD2 protein: an interaction with PRP11 defines a bridge between commitment complexes and U2 snRNP addition [3].

Biological context of PRP11

• Temperature-sensitive mutations in the genes RNA2 through RNA11 cause accumulation of intervening sequence containing precursor mRNAs in Saccharomyces cerevisiae [4].
• The yeast sequences on these plasmids have been shown by Southern transfer hybridization and genetic mapping to be derived from the RNA2, RNA3, and RNA11 genomic loci [4].
• Part of the RNA2 gene is homologous to more than one region of the yeast genome, whereas the RNA3 and RNA11 genes are single copy [4].

Anatomical context of PRP11

• RNA11 protein is associated with the yeast spliceosome and is localized in the periphery of the cell nucleus [5].

Other interactions of PRP11

• The yeast genes RNA2-RNA11 are necessary for splicing of nuclear intron-containing pre-mRNAs [6].
• Each of these spp mutations also suppresses prp3; spp41 and spp42 suppress prp11 as well [7].
• RNA blot-hybridization analyses coupled with genetic analyses indicated the RNA2+ is coded by a 3.0-kilobase (kb) transcript, RNA4+ is coded by a 1.6-kb transcript, and RNA11+ is coded by a 1.3-kb or a 1.7-kb transcript or both; none of the cloned genes contains detectable introns [8].

References