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Gene Review

TRM1  -  Trm1p

Saccharomyces cerevisiae S288c

Synonyms: YD9727.15C, YDR120C, tRNA (guanine(26)-N(2))-dimethyltransferase, mitochondrial, tRNA 2,2-dimethylguanosine-26 methyltransferase, tRNA(guanine-26,N(2)-N(2)) methyltransferase, ...
 
 
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Disease relevance of TRM1

 

High impact information on TRM1

 

Biological context of TRM1

  • An open reading frame in this TRM1 gene is essential for complementation of the trm1 phenotype [6].
  • The required identity elements in yeast tRNA for dimethylation under in vitro conditions by the yeast tRNA(m2(2)G26)-methyltransferase (the TRM1 gene product) are comprised of two G-C base pairs at positions G10-C25 and C11-G24 in the D-stem together with a variable loop of at least five nucleotides [7].
  • Comparatively short N- or C-terminal deletions from the 570 amino acid long Trm1 polypeptide decreased or eliminated the enzyme activity, as did some point mutations within these regions [8].
 

Associations of TRM1 with chemical compounds

  • Point and deletion mutations eliminate one or both methyl group transfers catalysed by the yeast TRM1 encoded tRNA (m22G26)dimethyltransferase [8].
 

Other interactions of TRM1

  • Other tRNA modification enzymes interacting with tRNA(Ser)CGA in the maturation process, such as Pus4p, Trm1 p, and Trm3p were essential or important for growth of the tRNA(Ser)CGA mutants [9].

References

  1. The tRNA(guanine-26,N2-N2) methyltransferase (Trm1) from the hyperthermophilic archaeon Pyrococcus furiosus: cloning, sequencing of the gene and its expression in Escherichia coli. Constantinesco, F., Benachenhou, N., Motorin, Y., Grosjean, H. Nucleic Acids Res. (1998) [Pubmed]
  2. N2,N2-dimethylguanosine-specific tRNA methyltransferase contains both nuclear and mitochondrial targeting signals in Saccharomyces cerevisiae. Li, J.M., Hopper, A.K., Martin, N.C. J. Cell Biol. (1989) [Pubmed]
  3. Amino-terminal extension generated from an upstream AUG codon is not required for mitochondrial import of yeast N2,N2-dimethylguanosine-specific tRNA methyltransferase. Ellis, S.R., Hopper, A.K., Martin, N.C. Proc. Natl. Acad. Sci. U.S.A. (1987) [Pubmed]
  4. Trm11p and Trm112p are both required for the formation of 2-methylguanosine at position 10 in yeast tRNA. Purushothaman, S.K., Bujnicki, J.M., Grosjean, H., Lapeyre, B. Mol. Cell. Biol. (2005) [Pubmed]
  5. Separate information required for nuclear and subnuclear localization: additional complexity in localizing an enzyme shared by mitochondria and nuclei. Rose, A.M., Joyce, P.B., Hopper, A.K., Martin, N.C. Mol. Cell. Biol. (1992) [Pubmed]
  6. Isolation and characterization of the TRM1 locus, a gene essential for the N2,N2-dimethylguanosine modification of both mitochondrial and cytoplasmic tRNA in Saccharomyces cerevisiae. Ellis, S.R., Morales, M.J., Li, J.M., Hopper, A.K., Martin, N.C. J. Biol. Chem. (1986) [Pubmed]
  7. Enzymatic formation of N2,N2-dimethylguanosine in eukaryotic tRNA: importance of the tRNA architecture. Edqvist, J., Stråby, K.B., Grosjean, H. Biochimie (1995) [Pubmed]
  8. Point and deletion mutations eliminate one or both methyl group transfers catalysed by the yeast TRM1 encoded tRNA (m22G26)dimethyltransferase. Liu, J., Liu, J., Stråby, K.B. Nucleic Acids Res. (1998) [Pubmed]
  9. Dual function of the tRNA(m(5)U54)methyltransferase in tRNA maturation. Johansson, M.J., Byström, A.S. RNA (2002) [Pubmed]
 
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