High impact information on TRP4

• Transcription from the yeast TRP4 promoter initiates at two basal (i127 and i76) and three GCN4 dependent (i31, i25 and i12) initiator elements [1].
• PHO2 binds specifically to the TRP4 promoter, but does not bind to any other TRP promoter [2].
• Exchanging the TRP4 3'UTR by the bidirectional ARO4 or the unidirectional GCN4 3' end formation element allowed efficient gene expression [3].
• A single point mutation within the TRP4 3'UTR caused the same effect on gene expression [3].
• A second region consists of the 3'-end of the TRP4 open reading frame and is required for 3'-end formation in forward orientation [4].

Biological context of TRP4

• The 3'UTR in reverse orientation was fully functional and did not affect TRP4 gene expression [3].
• A single point mutation in the yeast TRP4 gene affects efficiency of mRNA 3' end processing and alters selection of the poly(A) site [3].
• Promoter structures and spacing problems in the TRP4 promoter region are discussed [5].
• A 2 mu plasmid carrying the gene was integrated and mapped to chromosome IV, between trp4 and ade8, by the method of marker loss [6].

Associations of TRP4 with chemical compounds

• The TRP4 gene of Saccharomyces cerevisiae, which encodes anthranilate phosphoribosyl transferase (E.C. 2.4.2.18), is subject to the general control of amino acid biosynthesis [5].

Regulatory relationships of TRP4

• GCN4 might therefore activate transcription by exhibiting two alternative functions within the natural TRP4 promoter [7].

Other interactions of TRP4

• UAS1 is the major site for GCN4-dependent TRP4 activation [2].
• Conventional tetrad analysis has shown cdc40 to map 1.7 cM from trp4 [6].

References