Disease relevance of RPP2B

• On the other hand, there is very little apparent sequence similarity between YPA1 and the eubacterial acidic protein L12 either from E. coli or B. subtilis [1].

High impact information on RPP2B

• The mutated DNA was used to transform a yeast strain previously deprived of the original protein YP2 beta (L45) by gene disruption [2].
• By gene disruption techniques with linearized DNA from these constructions, strains of Saccharomyces cerevisiae were obtained which lacked a functional gene for either protein L44 or protein L45 [3].
• Proteins L44 and L45 have amino acid sequences with about 80% similarity [4].
• The genes coding for proteins L44 and L45 lack introns and seem also exceptional in other characteristics of their sequences [4].
• Some other rp-genes (e.g. those encoding S33 and L45) do not contain an RPG-box [5].

Biological context of RPP2B

• DNA probes from the genes encoding the acidic ribosomal proteins L44, L44' and L45, as well as from reporter genes for chromosomes IV, VII, XII and XV, have been hybridised to Southern blots of Saccharomyces cerevisiae DNA resolved by pulsed field gel electrophoresis [6].
• The replacement of each one of the eight serine residues present in the amino acid sequence of the Saccharomyces cerevisiae acidic ribosomal phosphoprotein YP2 beta (L45) by different amino acids has been performed by heteroduplex site-directed mutagenesis in the cloned gene [2].
• These results indicated that proteins L44 and L45 are independently dispensable for cell growth and that the ribosome is functional in the absence of either of them [3].
• The relevant nucleotide sequence, which is repeated in the L45-gene promoter, gives rise to complex formation with a yeast protein extract in a bandshift assay [7].
• We demonstrate that the CP1-site is not involved in the transcription activation of the L45-gene [7].

Associations of RPP2B with chemical compounds

• A comparison of protein YPA1 from yeast with eL12 from Artemia salina shows a high sequence similarity [1].

Physical interactions of RPP2B

• Replacement of this site by a consensus RAP1-binding site (an RPG box) did not alter the transcriptional yield of the L45-gene [7].

References