High impact information on VPS52

• Indeed, we find that Vps51p mediates the interaction between Vps52/53/54 and the t-SNARE Tlg1p [1].
• Vps51p mediates the association of the GARP (Vps52/53/54) complex with the late Golgi t-SNARE Tlg1p [1].
• Native coimmunoprecipitation experiments indicate that Vps52p, Vps53p, and Vps54p are associated in a 1:1:1 complex that sediments as a single peak on sucrose velocity gradients [2].
• Three of the genes (SAC1, SAC2 and SAC3) were subjected to extensive genetic and phenotypic analysis, including molecular cloning [3].
• A temperature-sensitive mutation (act1-1) in the essential actin gene of Saccharomyces cerevisiae can be suppressed by mutations in the SAC2 gene [4].

Biological context of VPS52

• A haploid SAC2 disruption strain failed to grow at low temperature and the disruption allele suppressed the temperature-sensitive act1-1 growth defect [4].
• Nucleotide sequence of the SAC2 gene of Saccharomyces cerevisiae [4].
• A SAC2 disruption mutation was constructed which had phenotypes similar to the sac2-1 point mutation [4].

Other interactions of VPS52

• Here we show that the Vps52/53/54 complex has an additional subunit, Vps51p [1].

References