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Gene Review:

MNN2 - Mnn2p

Saccharomyces cerevisiae S288c

Synonyms: Alpha-1,2-mannosyltransferase MNN2, CRV4, Calcium resistance and vanadate sensitivity protein 4, LDB8, Mannan synthesis protein MNN2, ...

Romero, P.A. et al., Nakamura, T. et al., Ibeas, J.I. et al., Olivero, I. et al., Corbacho, I. et al., et al.

Stocker, Tomizaki, Schulze-Briese, Baumann,

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High impact information on MNN2

We show here that full sensitivity of Saccharomyces cerevisiae cells to the PR-5 protein osmotin is dependent on the function of MNN2, MNN4 and MNN6 [1].
MNN2 is an alpha-1, 2-mannosyltransferase catalyzing the addition of the first mannose to the branches on the poly l,6-mannose backbone of the outer chain of cell wall N-linked mannans [1].
We conclude that the mnn2/ldb8 mutation is responsible for the inhibition of incorporation of phosphate to mannose A(3) (see below), a particular phosphorylation site of the inner core, while phosphorylation at the other possible site (mannose C(1)) is allowed, although it is also reduced. *Phosphorylation sites in mnn1mnn9 [2].
Deletion of CRV4/TTP1 causes no obvious phenotype except for Ca2+ resistance and vanadate sensitivity, but is synthetically lethal in combination with a deletion of MPK1, in a manner which is suppressible by the addition of an osmotic stabilizer [3].
We have cloned and characterized the CRV4 gene that complements all the phenotypes of the crv4 mutant [3].

Biological context of MNN2

The nucleotide sequence of TTP1, a gene encoding a predicted type II membrane protein [4].
Gene disruption indicated that TTP1 is not essential for cell growth [4].

Associations of MNN2 with chemical compounds

The product encoded by the gene, designated TTP1, is a predicted type II membrane protein of 597 amino acid residues with a short cytoplasmic NH2-terminus, a membrane-spanning region and a large COOH-terminal region containing three potential N-glycosylation sites [4].
SPF belongs to a family of lipid binding proteins called CRAL_TRIO, which include yeast phosphatidylinositol transfer protein Sec14 and tocopherol transfer protein TTP [5].

Other interactions of MNN2

The MNN1 family contains six members: MNN1, TTP1, YGL257c, YNR059w, YIL014w and YJL86w [6].

Analytical, diagnostic and therapeutic context of MNN2

In order to reveal possible interactions of MNN2 expression with other cellular pathways, we analyzed the transcriptome of the deletion mutant S. cerevisiae mnn2 Delta using cDNA microarrays [7].

References

Fungal cell wall phosphomannans facilitate the toxic activity of a plant PR-5 protein. Ibeas, J.I., Lee, H., Damsz, B., Prasad, D.T., Pardo, J.M., Hasegawa, P.M., Bressan, R.A., Narasimhan, M.L. Plant J. (2000) [Pubmed]
The mnn2 mutant of Saccharomyces cerevisiae is affected in phosphorylation of N-linked oligosaccharides. Olivero, I., Mañas, P., Hernández, L.M. FEBS Lett. (2000) [Pubmed]
Yeast Crv4/Ttp1, a predicted type II membrane protein, is involved in an event important for growth, functionally overlapping with the event regulated by calcineurin- and Mpk1-mediated pathways. Nakamura, T., Ohmoto, T., Hirata, D., Tsuchiya, E., Miyakawa, T. Mol. Gen. Genet. (1997) [Pubmed]
The nucleotide sequence of TTP1, a gene encoding a predicted type II membrane protein. Romero, P.A., Athanassiadis, A., Lussier, M., Herscovics, A. Yeast (1994) [Pubmed]
Crystal structure of the human supernatant protein factor. Stocker, A., Tomizaki, T., Schulze-Briese, C., Baumann, U. Structure (Camb.) (2002) [Pubmed]
The KTR and MNN1 mannosyltransferase families of Saccharomyces cerevisiae. Lussier, M., Sdicu, A.M., Bussey, H. Biochim. Biophys. Acta (1999) [Pubmed]
Genome-wide expression profile of the mnn2 Delta mutant of Saccharomyces cerevisiae. Corbacho, I., Olivero, I., Hohmann, S., Sunnerhagen, P., Hernández, L.M. Antonie Van Leeuwenhoek (2006) [Pubmed]

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AGOS_AER018C	Ashbya gossypii ATCC 10895
KLLA0E17755g	Kluyveromyces lactis NRRL Y-1140

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