Disease relevance of MET8

• Moreover, despite the fact that S. cerevisiae does not make cobalamins de novo, we have shown also that MET8 is able to complement cobalamin cobaltochelatase mutants and have revealed a subtle difference in the early stages of the anaerobic cobalamin biosynthetic pathways between Sal. typhimurium and Bacillus megaterium [1].

High impact information on MET8

• The DUR1,2 gene from Saccharomyces cerevisiae has been isolated on recombinant plasmids along with all DNA between the DUR1,2 and MET8 loci [2].
• Nucleotide sequence of the MET8 gene of Saccharomyces cerevisiae [3].
• The role of Saccharomyces cerevisiae Met1p and Met8p in sirohaem and cobalamin biosynthesis [1].
• The loci coding for these enzymes (dur1 and dur2) are very tightly linked on the right arm of chromosome II between pet11 and met8 [4].
• Met8p, from Saccharomyces cerevisiae, catalyses ferrochelation during the synthesis of sirohaem, and the structure reveals a novel chelatase architecture whereby both ferrochelation and NAD(+)-dependent dehydrogenation take place in a single bifunctional active site [5].

Other interactions of MET8

• Siroheme biosynthesis in Saccharomyces cerevisiae requires the products of both the MET1 and MET8 genes [6].

References