High impact information on PCA1

• Overexpression or deletion of the functional allele in yeast demonstrates that PCA1 is a cadmium efflux pump [1].
• The Yarrowia lipolytica gene PAY2 encodes a 42-kDa peroxisomal integral membrane protein essential for matrix protein import and peroxisome enlargement but not for peroxisome membrane proliferation [2].
• Pay2p is a peroxisomal integral membrane protein, as it localizes to carbonate-stripped peroxisomal membranes [2].
• An antibody raised against Pay2p recognizes a polypeptide of approximately 42-kDa whose synthesis is induced by growth of Y. lipolytica on oleic acid [2].
• The intracellular cadmium level of cells carrying CAD2 was lower than that of cells carrying either PAC1 or delta cad2 [3].

Biological context of PCA1

• The locus of CAD2 was mapped by gene linkage to a site 15.5 centimorgans to the right of the his7 locus on the right arm of chromosome II [4].

Associations of PCA1 with chemical compounds

• The cadmium-resistant gene, CAD2, which is a mutated putative copper-transporter gene (PCA1), controls the intracellular cadmium-level in the yeast S. cerevisiae [3].

Other interactions of PCA1

• The results suggest that the novel type of cadmium resistance controlled by CAD2 does ot involve production of a cadmium-metallothionein [4].

Analytical, diagnostic and therapeutic context of PCA1

• By site-directed mutagenesis, it appeared that the mutation conferring cadmium resistance was a R970G-substitution in the C-terminal region of Pca1 protein [3].
• Electron microscopy of pay2 cells revealed the presence of small peroxisomal "ghosts," similar to the vesicular structures found in fibroblasts of patients with the human peroxisome assembly disorder, Zellweger syndrome [2].

References