The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)



Gene Review

SUT1  -  Sut1p

Saccharomyces cerevisiae S288c

Synonyms: G1828, Sterol uptake protein 1, YGL162W
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

High impact information on SUT1

  • In contrast to SUT1, SUT4 is expressed predominantly in minor veins in source leaves, where high-capacity sucrose transport is needed for phloem loading [1].
  • In potato and tomato, SUT4 was immunolocalized specifically to enucleate sieve elements, indicating that like SUT1, macromolecular trafficking is required to transport the mRNA or the protein from companion cells through plasmodesmata into the sieve elements [1].
  • Examination of the subcellular distribution of sterols in these uptake mutants by cell fractionation and fluorescence microscopy indicates that some of the mutants block incorporation of cholesterol into the plasma membrane, a presumably early step in sterol uptake [2].
  • These results indicate that a hitherto uncharacterized mitochondrial function is required for sterol uptake and/or transport under anaerobic conditions and are discussed in light of the fact that mitochondrial import of cholesterol is required for steroidogenesis in vertebrate cells [2].
  • Ketoconazole exposure primarily affected genes involved in ergosterol biosynthesis and sterol uptake; caspofungin exposure affected genes involved in cell wall integrity; and 5-FC affected genes involved in DNA and protein synthesis, DNA damage repair, and cell cycle control [3].

Biological context of SUT1


Anatomical context of SUT1


Associations of SUT1 with chemical compounds

  • Unexpectedly, SUT1 constitutive expression led to a parallel significant increase in endogenous sterol biosynthesis [5].
  • In P. stipitis, transcription of SUT1 was strongly induced by glucose and was independent of the oxygen supply [9].
  • Similar to the sucrose transporter SUT1, StAAP1 expression was induced during the sink-to-source transition, indicating a role in phloem loading [10].
  • Sodium azide and vanadate inhibited sterol uptake, consistent with the participation of ATP-binding cassette transporters [8].
  • Regulation of ergosterol biosynthesis and sterol uptake in a sterol-auxotrophic yeast [11].

Physical interactions of SUT1

  • Furthermore, the N-terminal half of the low-affinity SUT2 interacts functionally with the C-terminal half of SUT1 [12].

Other interactions of SUT1

  • Within the promoter sequence of one of these genes, DAN1, we identified the region responsive to SUT1 and showed that it has a strong repressive activity when cloned in the vicinity of distinct promoters [4].
  • Moreover, here we present new data showing that the structurally related YPR009 gene (SUT2) is a functional homologue of SUT1, and that both gene products may represent two novel yeast regulatory proteins involved in sterol uptake [5].
  • We provide evidence that the repression is promoted by the Cyc8p(Ssn6p)-Tup1p co-repressor and that release of repression is the result of a physical interaction between Sut1p and Cyc8p [4].
  • We replaced the SUT1 promoter with the constitutive PMA1 gene promoter in order to enhance its transcription [5].
  • In addition, SUT1 effects on both sec14-1 suppression and on free sterol composition were abolished in a csr1-null background, showing that this gene acts downstream of SUT1 [6].

Analytical, diagnostic and therapeutic context of SUT1


  1. A new subfamily of sucrose transporters, SUT4, with low affinity/high capacity localized in enucleate sieve elements of plants. Weise, A., Barker, L., Kühn, C., Lalonde, S., Buschmann, H., Frommer, W.B., Ward, J.M. Plant Cell (2000) [Pubmed]
  2. A genomewide screen reveals a role of mitochondria in anaerobic uptake of sterols in yeast. Reiner, S., Micolod, D., Zellnig, G., Schneiter, R. Mol. Biol. Cell (2006) [Pubmed]
  3. Genome-wide expression profiling of the response to polyene, pyrimidine, azole, and echinocandin antifungal agents in Saccharomyces cerevisiae. Agarwal, A.K., Rogers, P.D., Baerson, S.R., Jacob, M.R., Barker, K.S., Cleary, J.D., Walker, L.A., Nagle, D.G., Clark, A.M. J. Biol. Chem. (2003) [Pubmed]
  4. SUT1p interaction with Cyc8p(Ssn6p) relieves hypoxic genes from Cyc8p-Tup1p repression in Saccharomyces cerevisiae. Régnacq, M., Alimardani, P., El Moudni, B., Bergès, T. Mol. Microbiol. (2001) [Pubmed]
  5. SUT1 is a putative Zn[II]2Cys6-transcription factor whose upregulation enhances both sterol uptake and synthesis in aerobically growing Saccharomyces cerevisiae cells. Ness, F., Bourot, S., Régnacq, M., Spagnoli, R., Bergès, T., Karst, F. Eur. J. Biochem. (2001) [Pubmed]
  6. SUT1 suppresses sec14-1 through upregulation of CSR1 in Saccharomyces cerevisiae. Régnacq, M., Ferreira, T., Puard, J., Bergès, T. FEMS Microbiol. Lett. (2002) [Pubmed]
  7. SUT1-promoted sterol uptake involves the ABC transporter Aus1 and the mannoprotein Dan1 whose synergistic action is sufficient for this process. Alimardani, P., Régnacq, M., Moreau-Vauzelle, C., Ferreira, T., Rossignol, T., Blondin, B., Bergès, T. Biochem. J. (2004) [Pubmed]
  8. Transcriptional profiling identifies two members of the ATP-binding cassette transporter superfamily required for sterol uptake in yeast. Wilcox, L.J., Balderes, D.A., Wharton, B., Tinkelenberg, A.H., Rao, G., Sturley, S.L. J. Biol. Chem. (2002) [Pubmed]
  9. Cloning and characterization of three genes (SUT1-3) encoding glucose transporters of the yeast Pichia stipitis. Weierstall, T., Hollenberg, C.P., Boles, E. Mol. Microbiol. (1999) [Pubmed]
  10. Reduced amino acid content in transgenic potato tubers due to antisense inhibition of the leaf H+/amino acid symporter StAAP1. Koch, W., Kwart, M., Laubner, M., Heineke, D., Stransky, H., Frommer, W.B., Tegeder, M. Plant J. (2003) [Pubmed]
  11. Regulation of ergosterol biosynthesis and sterol uptake in a sterol-auxotrophic yeast. Lorenz, R.T., Parks, L.W. J. Bacteriol. (1987) [Pubmed]
  12. Intra- and intermolecular interactions in sucrose transporters at the plasma membrane detected by the split-ubiquitin system and functional assays. Reinders, A., Schulze, W., Thaminy, S., Stagljar, I., Frommer, W.B., Ward, J.M. Structure (Camb.) (2002) [Pubmed]
  13. Isolation and characterization of the Saccharomyces cerevisiae SUT1 gene involved in sterol uptake. Bourot, S., Karst, F. Gene (1995) [Pubmed]
  14. Identification of a UPC2 homolog in Saccharomyces cerevisiae and its involvement in aerobic sterol uptake. Shianna, K.V., Dotson, W.D., Tove, S., Parks, L.W. J. Bacteriol. (2001) [Pubmed]
WikiGenes - Universities