Disease relevance of ARO2

• The ARO2 gene product is highly similar to its Escherichia coli counterpart, with a 47% identity distributed over the entire length of the peptide [1].

High impact information on ARO2

• To examine the chromosomal stability of repetitions of the trinucleotide CAG, we have cloned CAG repeat tracts onto the 3' end of the Saccharomyces cerevisiae ADE2 gene and placed the appended gene into the ARO2 locus of chromosome VII [2].
• The chorismate synthase reaction involves a 1,4-trans-elimination of phosphoric acid from EPSP and has an absolute requirement for reduced FMN as a cofactor [3].
• We have determined the three-dimensional x-ray structure of the yeast chorismate synthase from selenomethionine-labeled crystals at 2.2-A resolution [3].
• Chorismate synthase (EC 4.2.3.5), the seventh enzyme in the shikimate pathway, catalyzes the transformation of 5-enolpyruvylshikimate 3-phosphate (EPSP) to chorismate, which is the last common precursor in the biosynthesis of numerous aromatic compounds in bacteria, fungi, and plants [3].
• ARO2 is regulated under the 'general control system' for amino acid biosynthesis by the transcriptional activator GCN4 which binds in vitro at three sites within the ARO2 promoter [1].

Biological context of ARO2

• The inhibition of sporulation seems to occur at a very early step in meiosis, as indicated by the lack of premeiotic DNA synthesis in aro1 and aro2 mutants [4].

Associations of ARO2 with chemical compounds

• ARO2 codes for chorismate synthetase [5].

Other interactions of ARO2

• Mutations in ARO1 and ARO2 genes coding for enzymes involved in the common part of the aromatic amino acid pathway completely block the sporulation of Saccharomyces cerevisiae when in a homozygous state, whereas mutations in all the other genes of the same pathway do not [4].

References