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Gene Review

CDC20  -  Cdc20p

Saccharomyces cerevisiae S288c

Synonyms: APC/C activator protein CDC20, Cell division control protein 20, YGL116W
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High impact information on CDC20


Biological context of CDC20

  • Overexpression of either CDC20 or CDH1 was sufficient to induce APC-dependent proteolysis of the appropriate target in stages of the cell cycle in which substrates are normally stable [6].
  • In Saccharomyces cerevisiae, chromosome segregation also requires the CDC20 gene, whose product contains WD40 repeats [11,12] [7].
  • CDC20 overexpression was unable to promote anaphase in cells deficient in APC function [8].
  • Therefore, we suggest that the DNA damage-induced checkpoint controls prevent mitosis by inhibiting the nuclear division pathway requiring CDC20 function [9].
  • Cdc20, an activator of the anaphase-promoting complex (APC), is also required for the exit from mitosis in Saccharomyces cerevisiae [10].

Anatomical context of CDC20


Associations of CDC20 with chemical compounds

  • Here we find that the cyclic-AMP-dependent protein kinase (PKA) pathway supports Chk1 in the regulation of mitosis by targeting the mitotic inducer Cdc20 [15].

Physical interactions of CDC20

  • The checkpoint proteins Mad2 and Mad3/BubR1 bind to Cdc20, although how they inhibit APC(Cdc20) is unclear [16].
  • Accumulation of Mad2-Cdc20 complex during spindle checkpoint activation requires binding of open and closed conformers of Mad2 in Saccharomyces cerevisiae [17].
  • Finally, this bacterially expressed Hsl1p fusion protein interacted with Cdc20p and Cdh1p either translated in vitro or expressed in and purified from insect cells [18].
  • Here we find that Pds1 interacts directly with Cdc20 and that this interaction requires Pds1's destruction box [19].

Regulatory relationships of CDC20

  • Phosphorylation by Cdc28 activates the Cdc20-dependent activity of the anaphase-promoting complex [20].
  • Overproduction of Cdc20 does not influence the arrest phenotype of the cdc mutants whose cell cycle block is independent of RAD9-mediated checkpoint control [9].
  • Cdc20p may promote the APC-dependent proteolytic degradation of Pds1p and other factors that act to inhibit cell cycle progression through mitosis [8].
  • Although the precise mechanism of APC inhibition by Cla4t remains to be elucidated, our results suggest that Cla4 and Ste20 may regulate the first wave of cyclinB proteolysis mediated by Cdc20/APC, which has been shown to be crucial for activation of the mitotic exit network (MEN) [21].
  • The WD repeat protein Cdc20 is essential for progression through mitosis because it is required to activate ubiquitin ligation by the anaphase-promoting complex (APC/C) [22].

Other interactions of CDC20

  • CDC20 and CDH1: a family of substrate-specific activators of APC-dependent proteolysis [6].
  • These findings implicate Mad3p as a pseudosubstrate inhibitor of APC(Cdc20), competing with APC substrates for Cdc20p binding [16].
  • Cyclin-specific APC activity in cells overexpressing CDC5 was reduced in the absence of the APC regulatory proteins Hct 1 and Cdc20 [23].
  • Furthermore, the APC activator Cdc20 is itself a substrate of the Cdc27 have a role in the degradation of Cdc20 during S Phase and early mitosis that is not mediated by its destruction box [24].
  • The regulation of Cdc20 proteolysis reveals a role for APC components Cdc23 and Cdc27 during S phase and early mitosis [24].

Analytical, diagnostic and therapeutic context of CDC20


  1. How do so few control so many? Nasmyth, K. Cell (2005) [Pubmed]
  2. Emi1 is a mitotic regulator that interacts with Cdc20 and inhibits the anaphase promoting complex. Reimann, J.D., Freed, E., Hsu, J.Y., Kramer, E.R., Peters, J.M., Jackson, P.K. Cell (2001) [Pubmed]
  3. Yeast Hct1 is a regulator of Clb2 cyclin proteolysis. Schwab, M., Lutum, A.S., Seufert, W. Cell (1997) [Pubmed]
  4. APC-dependent proteolysis of the mitotic cyclin Clb2 is essential for mitotic exit. Wäsch, R., Cross, F.R. Nature (2002) [Pubmed]
  5. Budding yeast Cdc20: a target of the spindle checkpoint. Hwang, L.H., Lau, L.F., Smith, D.L., Mistrot, C.A., Hardwick, K.G., Hwang, E.S., Amon, A., Murray, A.W. Science (1998) [Pubmed]
  6. CDC20 and CDH1: a family of substrate-specific activators of APC-dependent proteolysis. Visintin, R., Prinz, S., Amon, A. Science (1997) [Pubmed]
  7. Cdc20 is essential for the cyclosome-mediated proteolysis of both Pds1 and Clb2 during M phase in budding yeast. Lim, H.H., Goh, P.Y., Surana, U. Curr. Biol. (1998) [Pubmed]
  8. Dominant alleles of Saccharomyces cerevisiae CDC20 reveal its role in promoting anaphase. Schott, E.J., Hoyt, M.A. Genetics (1998) [Pubmed]
  9. Cdc20, a beta-transducin homologue, links RAD9-mediated G2/M checkpoint control to mitosis in Saccharomyces cerevisiae. Lim, H.H., Surana, U. Mol. Gen. Genet. (1996) [Pubmed]
  10. Exit from mitosis in budding yeast: biphasic inactivation of the Cdc28-Clb2 mitotic kinase and the role of Cdc20. Yeong, F.M., Lim, H.H., Padmashree, C.G., Surana, U. Mol. Cell (2000) [Pubmed]
  11. Spindle checkpoint regulates Cdc20p stability in Saccharomyces cerevisiae. Pan, J., Chen, R.H. Genes Dev. (2004) [Pubmed]
  12. Rapid microtubule-independent dynamics of Cdc20 at kinetochores and centrosomes in mammalian cells. Kallio, M.J., Beardmore, V.A., Weinstein, J., Gorbsky, G.J. J. Cell Biol. (2002) [Pubmed]
  13. Cdc20 associates with the kinase aurora2/Aik. Farruggio, D.C., Townsley, F.M., Ruderman, J.V. Proc. Natl. Acad. Sci. U.S.A. (1999) [Pubmed]
  14. Cortex, a Drosophila gene required to complete oocyte meiosis, is a member of the Cdc20/fizzy protein family. Chu, T., Henrion, G., Haegeli, V., Strickland, S. Genesis (2001) [Pubmed]
  15. The DNA damage checkpoint and PKA pathways converge on APC substrates and Cdc20 to regulate mitotic progression. Searle, J.S., Schollaert, K.L., Wilkins, B.J., Sanchez, Y. Nat. Cell Biol. (2004) [Pubmed]
  16. Mad3p, a pseudosubstrate inhibitor of APCCdc20 in the spindle assembly checkpoint. Burton, J.L., Solomon, M.J. Genes Dev. (2007) [Pubmed]
  17. Accumulation of Mad2-Cdc20 complex during spindle checkpoint activation requires binding of open and closed conformers of Mad2 in Saccharomyces cerevisiae. Nezi, L., Rancati, G., De Antoni, A., Pasqualato, S., Piatti, S., Musacchio, A. J. Cell Biol. (2006) [Pubmed]
  18. D box and KEN box motifs in budding yeast Hsl1p are required for APC-mediated degradation and direct binding to Cdc20p and Cdh1p. Burton, J.L., Solomon, M.J. Genes Dev. (2001) [Pubmed]
  19. The anaphase inhibitor Pds1 binds to the APC/C-associated protein Cdc20 in a destruction box-dependent manner. Hilioti, Z., Chung, Y.S., Mochizuki, Y., Hardy, C.F., Cohen-Fix, O. Curr. Biol. (2001) [Pubmed]
  20. Phosphorylation by Cdc28 activates the Cdc20-dependent activity of the anaphase-promoting complex. Rudner, A.D., Murray, A.W. J. Cell Biol. (2000) [Pubmed]
  21. Budding yeast PAK kinases regulate mitotic exit by two different mechanisms. Chiroli, E., Fraschini, R., Beretta, A., Tonelli, M., Lucchini, G., Piatti, S. J. Cell Biol. (2003) [Pubmed]
  22. The CCT chaperonin promotes activation of the anaphase-promoting complex through the generation of functional Cdc20. Camasses, A., Bogdanova, A., Shevchenko, A., Zachariae, W. Mol. Cell (2003) [Pubmed]
  23. The Polo-related kinase Cdc5 activates and is destroyed by the mitotic cyclin destruction machinery in S. cerevisiae. Charles, J.F., Jaspersen, S.L., Tinker-Kulberg, R.L., Hwang, L., Szidon, A., Morgan, D.O. Curr. Biol. (1998) [Pubmed]
  24. The regulation of Cdc20 proteolysis reveals a role for APC components Cdc23 and Cdc27 during S phase and early mitosis. Prinz, S., Hwang, E.S., Visintin, R., Amon, A. Curr. Biol. (1998) [Pubmed]
  25. The CDC20 gene product of Saccharomyces cerevisiae, a beta-transducin homolog, is required for a subset of microtubule-dependent cellular processes. Sethi, N., Monteagudo, M.C., Koshland, D., Hogan, E., Burke, D.J. Mol. Cell. Biol. (1991) [Pubmed]
  26. Structure of the Mad2 spindle assembly checkpoint protein and its interaction with Cdc20. Luo, X., Fang, G., Coldiron, M., Lin, Y., Yu, H., Kirschner, M.W., Wagner, G. Nat. Struct. Biol. (2000) [Pubmed]
  27. Identification of an overlapping binding domain on Cdc20 for Mad2 and anaphase-promoting complex: model for spindle checkpoint regulation. Zhang, Y., Lees, E. Mol. Cell. Biol. (2001) [Pubmed]
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