Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

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Gene Review:

ALG13 - N-acetylglucosaminyldiphosphodolichol N...

Saccharomyces cerevisiae S288c

Synonyms: Asparagine-linked glycosylation protein 13, UDP-N-acetylglucosamine transferase subunit ALG13, YGL047W

Bickel, T. et al., Gao, X.D. et al., Gao, X.D. et al.

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High impact information on ALG13

Microsomal membranes from cells repressed for ALG13 or ALG14, as well as detergent-solubilized extracts thereof, were unable to catalyze the transfer of N-acetylglucosamine from UDP-GlcNAc to [(14)C]GlcNAc(1)-PP-Dol, but did not impair the formation of GlcNAc(1)-PP-Dol or GlcNAc-GPI [1].
Alg13 contains a predicted catalytic domain, but lacks any membrane-spanning domains [2].
In Western blot analysis it was demonstrated that Alg13p, for which no well defined transmembrane segment has been predicted, localizes both to the membrane and cytosol; the latter form, however, is enzymatically inactive [1].
Alg14 recruits Alg13 to the cytoplasmic face of the endoplasmic reticulum to form a novel bipartite UDP-N-acetylglucosamine transferase required for the second step of N-linked glycosylation [2].

Physical interactions of ALG13

Electrostatic surface views of the modeled Alg13/14 complex suggest the presence of a hydrophobic cleft in Alg14 that provides a pocket for the Alg13 C-terminal alpha-helix [3].

References

Biosynthesis of lipid-linked oligosaccharides in Saccharomyces cerevisiae: Alg13p and Alg14p form a complex required for the formation of GlcNAc(2)-PP-dolichol. Bickel, T., Lehle, L., Schwarz, M., Aebi, M., Jakob, C.A. J. Biol. Chem. (2005) [Pubmed]
Alg14 recruits Alg13 to the cytoplasmic face of the endoplasmic reticulum to form a novel bipartite UDP-N-acetylglucosamine transferase required for the second step of N-linked glycosylation. Gao, X.D., Tachikawa, H., Sato, T., Jigami, Y., Dean, N. J. Biol. Chem. (2005) [Pubmed]
Interaction between the C termini of Alg13 and Alg14 mediates formation of the active UDP-N-acetylglucosamine transferase complex. Gao, X.D., Moriyama, S., Miura, N., Dean, N., Nishimura, S. J. Biol. Chem. (2008) [Pubmed]

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