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Gene Review:

ASN2 - asparagine synthase (glutamine-hydrolyzing) 2

Saccharomyces cerevisiae S288c

Synonyms: G6358, Glutamine-dependent asparagine synthetase 2, YGR124W

Dang, V.D. et al., Lam, H.M. et al., Zimmer, T. et al., Jones, G.E.

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High impact information on ASN2

Two new yeast genes, named ASN1 and ASN2, were isolated by complementation of the growth defect of an asparagine auxotrophic mutant [1].
Regulation of ASN1 and ASN2 expression was studied using lacZ fusions and both genes were found to be several times less expressed in the absence of the transcription activator Gcn4p [1].
The proteins encoded by the ASN2/3 cDNAs contain a Pur-F type glutamine-binding triad suggesting that they, like ASN1, encode glutamine-dependent asparagine synthetase isoenzymes [2].
Using the mRNA differential display technique, six genes were found to be up-regulated: ASN2, MDJ1, YLR194c, YNL208w, YER175, and YGL121c [3].

Biological context of ASN2

Nucleotide sequences of ASN1 and ASN2 revealed striking similarities with genes encoding asparagine synthetase (AS) from other organisms [1].
L-Asparagine auxotrophy in Saccharomyces cerevisiae is the result of mutation in each of two unlinked cistrons, ASN1 and ASN2 [4].

Regulatory relationships of ASN2

Time-course experiments reveal that light induces levels of ASN2 mRNA while it represses levels of ASN1 mRNA in a kinetically reciprocal fashion [2].

References

Cloning of the ASN1 and ASN2 genes encoding asparagine synthetases in Saccharomyces cerevisiae: differential regulation by the CCAAT-box-binding factor. Dang, V.D., Valens, M., Bolotin-Fukuhara, M., Daignan-Fornier, B. Mol. Microbiol. (1996) [Pubmed]
Reciprocal regulation of distinct asparagine synthetase genes by light and metabolites in Arabidopsis thaliana. Lam, H.M., Hsieh, M.H., Coruzzi, G. Plant J. (1998) [Pubmed]
Gene regulation in response to overexpression of cytochrome P450 and proliferation of the endoplasmic reticulum in Saccharomyces cerevisiae. Zimmer, T., Ogura, A., Takewaka, T., Zimmer, R.M., Ohta, A., Takagi, M. Biosci. Biotechnol. Biochem. (2000) [Pubmed]
L-Asparagine auxotrophs of Saccharomyces cerevisiae: genetic and phenotypic characterization. Jones, G.E. J. Bacteriol. (1978) [Pubmed]

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ASN3	Arabidopsis thaliana
ASN2	Arabidopsis thaliana
MGG_00969	Magnaporthe oryzae 70-15
NCU04303	Neurospora crassa OR74A
Os06g0265000	Oryza sativa Japonica Group
ASN1	Saccharomyces cerevisiae S288c
asn1	Schizosaccharomyces pombe 972h-

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