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Gene Review

EAP1  -  Eap1p

Saccharomyces cerevisiae S288c

Synonyms: Protein EAP1, YKL204W, eIF4E-associated protein 1
 
 
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High impact information on EAP1

  • Using distinct vesicular transport mutants and chlorpromazine, we have identified the eIF2alpha kinase Gcn2p and the eIF4E binding protein Eap1p as major mediators of the translation attenuation response [1].
  • Targeted disruption of the EAP1 gene results in a temperature-sensitive phenotype and also confers partial resistance to growth inhibition by rapamycin [2].
  • Deletion of this domain or point mutation abolishes the interaction of Eap1p with eIF4E [2].
  • These data indicate that Eap1p plays a role in cell growth and implicates this protein in the TOR signaling cascade of S. cerevisiae [2].
  • Here, we characterized the genetic interactions between EAP1 and NDC1, a gene whose function is required for duplication of the spindle pole body (SPB) [12], the centrosome-equivalent organelle in yeast that functions as the centrosome [3].
 

Biological context of EAP1

  • To reconfirm scp160/eap1 synthetic lethality, we constructed a strain null for both genes, supported by an SCP160 maintenance plasmid [4].
  • We have found that disruption of the genes for either of the yeast 4E-BPs (Eap1p or Caf20p) leads to an inhibition of pseudohyphal growth in the resulting diploid yeast strain following nitrogen limitation [5].
 

Anatomical context of EAP1

 

Associations of EAP1 with chemical compounds

  • Transcription of EAP1 in C. albicans is regulated by the transcription factor Efg1p, suggesting that EAP1 expression is activated by the cyclic AMP-dependent protein kinase pathway [6].
  • In addition to increasing adhesion to polystyrene, heterologous expression of EAP1 in S. cerevisiae and autonomous expression of EAP1 in a C. albicans efg1 homozygous null mutant significantly enhanced attachment to HEK293 kidney epithelial cells [6].
 

Physical interactions of EAP1

  • In yeast, EAP1 encodes a protein that binds eIF4E and inhibits cap-dependent translation in vitro [11] [3].
 

Other interactions of EAP1

  • Finally, we probed the impact of EAP1 loss on Scp160p, and vice versa, and found that loss of each gene resulted in a significant change in either the complex associations or subcellular distribution of the other protein [4].
  • The yeast eIF4E-associated protein Eap1p attenuates GCN4 translation upon TOR-inactivation [7].
 

Analytical, diagnostic and therapeutic context of EAP1

  • Sequence analysis of EAP1 shows that it contains a signal peptide, a glycosylphosphatidylinositol anchor site, and possesses homology to many other yeast genes encoding cell wall proteins [6].

References

  1. A membrane transport defect leads to a rapid attenuation of translation initiation in Saccharomyces cerevisiae. Deloche, O., de la Cruz, J., Kressler, D., Doère, M., Linder, P. Mol. Cell (2004) [Pubmed]
  2. Eap1p, a novel eukaryotic translation initiation factor 4E-associated protein in Saccharomyces cerevisiae. Cosentino, G.P., Schmelzle, T., Haghighat, A., Helliwell, S.B., Hall, M.N., Sonenberg, N. Mol. Cell. Biol. (2000) [Pubmed]
  3. Yeast Eap1p, an eIF4E-associated protein, has a separate function involving genetic stability. Chial, H.J., Stemm-Wolf, A.J., McBratney, S., Winey, M. Curr. Biol. (2000) [Pubmed]
  4. Genetic and biochemical interactions between SCP160 and EAP1 in yeast. Mendelsohn, B.A., Li, A.M., Vargas, C.A., Riehman, K., Watson, A., Fridovich-Keil, J.L. Nucleic Acids Res. (2003) [Pubmed]
  5. Regulation of translation initiation by the yeast eIF4E binding proteins is required for the pseudohyphal response. Ibrahimo, S., Holmes, L.E., Ashe, M.P. Yeast (2006) [Pubmed]
  6. EAP1, a Candida albicans gene involved in binding human epithelial cells. Li, F., Palecek, S.P. Eukaryotic Cell (2003) [Pubmed]
  7. The yeast eIF4E-associated protein Eap1p attenuates GCN4 translation upon TOR-inactivation. Matsuo, R., Kubota, H., Obata, T., Kito, K., Ota, K., Kitazono, T., Ibayashi, S., Sasaki, T., Iida, M., Ito, T. FEBS Lett. (2005) [Pubmed]
 
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