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UGP1  -  UTP glucose-1-phosphate uridylyltransferase

Saccharomyces cerevisiae S288c

Synonyms: UDP-glucose pyrophosphorylase, UDPGP, UGPase, UTP--glucose-1-phosphate uridylyltransferase, YKL035W, ...
 
 
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Disease relevance of UGP1

 

High impact information on UGP1

  • PAS kinase was also found to phosphorylate UDP-glucose pyrophosphorylase and glycogen synthase, the enzymes catalyzing the two final steps in the glycogen biosynthetic pathway [4].
  • A new active site directed photoaffinity probe, which is a model compound for studying nucleotide diphosphate sugar binding proteins, has been synthesized by coupling 5-azido-UTP and [32P]Glc-1-P using yeast UDP-glucose pyrophosphorylase to produce [beta-32P]5-azidouridine 5'-diphosphoglucose (5N3UDP-Glc) [5].
  • We propose that GalF is a non-catalytic subunit of the UDP-glucose pyrophosphorylase modulating the enzyme activity to increase the formation of UDP-glucose, and this function is important for bacterial adaptation to conditions of stress [2].
  • The catalytic domains attached to this scaffold core do not directly contact each other, consistent with simple Michaelis-Menten kinetics found for Ugp1p [6].
  • Quantification of uridine 5'-diphosphate (UDP)-glucose by high-performance liquid chromatography and its application to a nonradioactive assay for nucleoside diphosphate kinase using UDP-glucose pyrophosphorylase as a coupling enzyme [7].
 

Biological context of UGP1

  • Taken altogether, these results indicate that a few percent of enzymatic activities leading to the formation of UDP-Glc appears sufficient to provide the UDP-Glc demands required for cell viability, and that the loss of function of UGP1 is lethal mainly because of the inability of yeast cells to properly form the cell wall [8].
  • The phenotypic effects of this reduction were slightly stronger than those of glucose on the GALI0/CYC1-UGP1 gene fusion [Daran et al. (1995) Eur. J. Biochem. 230, 520-530] [8].
  • This was done by generating haploid strains carrying either UGP1 on a multicopy vector or the chromosomal deletion of UGP1, and rescued by a vector bearing the wild-type gene under the control of the glucose-repressible galactose-inducible promoter [9].
  • To test if augmented expression of this gene could produce a Gal(+) phenotype in the GALT-deficient parent cells, we overexpressed the yeast UGP1 and the human homolog, hUGP2 in the mutant strain [10].
  • The sequence of a 12 kb fragment on the left arm of yeast chromosome XI reveals five new open reading frames, including a zinc finger protein and a homolog of the UDP-glucose pyrophosphorylase from potato [11].
 

Associations of UGP1 with chemical compounds

 

Other interactions of UGP1

  • We found that expression of various genes involved in carbon metabolism are affected by the mutation and that among them, UGP1 promoter activity was increased in the absence of Pho85 kinase [12].
  • Firstly, UGP1 was placed under the strongly regulatable THI4 promoter [8].
  • We have detected five new open reading frames (ORFs) longer than 300 bp provisionally called YKL248 to YKL252 [11].
  • A further reduction of flux towards UDP-Glc was achieved by deletion of the two phosphoglucomutase genes in the ugp1 conditional strain [8].

References

  1. Expression of human inositol monophosphatase suppresses galactose toxicity in Saccharomyces cerevisiae: possible implications in galactosemia. Mehta, D.V., Kabir, A., Bhat, P.J. Biochim. Biophys. Acta (1999) [Pubmed]
  2. The GalF protein of Escherichia coli is not a UDP-glucose pyrophosphorylase but interacts with the GalU protein possibly to regulate cellular levels of UDP-glucose. Marolda, C.L., Valvano, M.A. Mol. Microbiol. (1996) [Pubmed]
  3. Bacillus subtilis gtaB encodes UDP-glucose pyrophosphorylase and is controlled by stationary-phase transcription factor sigma B. Varón, D., Boylan, S.A., Okamoto, K., Price, C.W. J. Bacteriol. (1993) [Pubmed]
  4. Coordinate regulation of sugar flux and translation by PAS kinase. Rutter, J., Probst, B.L., McKnight, S.L. Cell (2002) [Pubmed]
  5. Synthesis and properties of 5-azido-UDP-glucose. Development of photoaffinity probes for nucleotide diphosphate sugar binding sites. Drake, R.R., Evans, R.K., Wolf, M.J., Haley, B.E. J. Biol. Chem. (1989) [Pubmed]
  6. Structural Basis for Subunit Assembly in UDP-glucose Pyrophosphorylase from Saccharomyces cerevisiae. Roeben, A., Plitzko, J.M., K??rner, R., B??ttcher, U.M., Siegers, K., Hayer-Hartl, M., Bracher, A. J. Mol. Biol. (2006) [Pubmed]
  7. Quantification of uridine 5'-diphosphate (UDP)-glucose by high-performance liquid chromatography and its application to a nonradioactive assay for nucleoside diphosphate kinase using UDP-glucose pyrophosphorylase as a coupling enzyme. Dorion, S., Rivoal, J. Anal. Biochem. (2003) [Pubmed]
  8. Physiological and morphological effects of genetic alterations leading to a reduced synthesis of UDP-glucose in Saccharomyces cerevisiae. Daran, J.M., Bell, W., François, J. FEMS Microbiol. Lett. (1997) [Pubmed]
  9. Genetic and biochemical characterization of the UGP1 gene encoding the UDP-glucose pyrophosphorylase from Saccharomyces cerevisiae. Daran, J.M., Dallies, N., Thines-Sempoux, D., Paquet, V., François, J. Eur. J. Biochem. (1995) [Pubmed]
  10. Overexpression of human UDP-glucose pyrophosphorylase rescues galactose-1-phosphate uridyltransferase-deficient yeast. Lai, K., Elsas, L.J. Biochem. Biophys. Res. Commun. (2000) [Pubmed]
  11. The sequence of a 12 kb fragment on the left arm of yeast chromosome XI reveals five new open reading frames, including a zinc finger protein and a homolog of the UDP-glucose pyrophosphorylase from potato. Purnelle, B., Skala, J., Van Dyck, L., Goffeau, A. Yeast (1992) [Pubmed]
  12. Pho85 kinase, a yeast cyclin-dependent kinase, regulates the expression of UGP1 encoding UDP-glucose pyrophosphorylase. Nishizawa, M., Tanabe, M., Yabuki, N., Kitada, K., Toh-E, A. Yeast (2001) [Pubmed]
 
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