The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
Gene Review

SUP3  -  tRNA

Saccharomyces cerevisiae S288c

 
 
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.
 

High impact information on SUP3

  • Successive rounds of mutagenesis of a Schizosaccharomyces pombe strain bearing the UGA-reading sup3 tRNASer suppressor have been carried out for two cycles of inactivation and reactivation of the suppressor [1].
  • Using these methods we have determined the sequence of a 410 base pair fragment which includes the yeast SUP3 tyrosine tRNA gene [2].
  • Different levels of inhibition were found ranging from SUP3-omicron, which caused the greatest reduction of sporulation (7-17% of wild type), to SUP11-omicron which caused no reduction in sporulation [3].
  • SUP3-omicron/SUP3-omicron diploid cells transferred from log or early stationary phase are capable of sporulation, whereas cells transferred after early stationary phase (i.e., after adaptation to respiration) exhibit poor sporulative ability [3].
  • A meiotic fine structure map of a yeast tyrosine-inserting ochre suppressor, SUP3-omicron, was constructed [4].
 

Biological context of SUP3

  • SCM2 is located on the left arm of yeast chromosome XV, adjacent to SUP3 and encodes a 65 kDa protein that is highly homologous to known amino acid permeases [5].
  • Simultaneous amber and ochre suppression with the suppressors SUP3 and SUP11, respectively, was capable of relieving the tryptophan-requiring phenotype of strains carrying the trp5-27 allele [6].
  • The spd1 locus has been mapped 13.9 cM to the left of the centromere on chromosome XV, adjacent to the SUP3 gene [7].
  • It has no effect on the stability of the nonsense alleles arg1-230 (UAA), ade6-704 and ural1-61 (UGA) or on the frequency of crossing-over between sup3 and the closely linked gene cdc8 [8].
  • The existence of a common genetic control over intragenic recombination and genetic instability at sup3 provides a direct way of selecting for rec mutants in homothallic haploid strains of S. pombe carrying a suppressor-inactive allele of sup3 [8].
 

Other interactions of SUP3

 

Analytical, diagnostic and therapeutic context of SUP3

  • Sequence analysis of these revertant alleles corroborates genetic evidence for mutational hotspots within the sup3 tRNA gene [11].

References

  1. Functional complementation between mutations in a yeast suppressor tRNA gene reveals potential for evolution of tRNA sequences. Willis, I., Nichols, M., Chisholm, V., Söll, D., Heyer, W.D., Szankasi, P., Amstutz, H., Munz, P., Kohli, J. Proc. Natl. Acad. Sci. U.S.A. (1986) [Pubmed]
  2. New rapid methods for DNA sequencing based in exonuclease III digestion followed by repair synthesis. Guo, L.H., Wu, R. Nucleic Acids Res. (1982) [Pubmed]
  3. The effect of ochre suppression on meiosis and ascospore formation in Saccharomyces. Rothstein, R.J., Esposito, R.E., Esposito, M.S. Genetics (1977) [Pubmed]
  4. A genetic fine structure analysis of the suppressor 3 locus in Saccharomyces. Rothstein, R.J. Genetics (1977) [Pubmed]
  5. SCM2, a tryptophan permease in Saccharomyces cerevisiae, is important for cell growth. Chen, X.H., Xiao, Z., Fitzgerald-Hayes, M. Mol. Gen. Genet. (1994) [Pubmed]
  6. Characterization of the trp5-27 allele used to monitor drug-induced mitotic gene conversion in the Saccharomyces cerevisiae tester strain D7. Ehrenhofer-Murray, A.E., Würgler, F.E., Sengstag, C. Mutagenesis (1994) [Pubmed]
  7. Initiation of sporulation in Saccharomyces cerevisiae. Mutations causing derepressed sporulation and G1 arrest in the cell division cycle. Dawes, I.W., Calvert, G.R. J. Gen. Microbiol. (1984) [Pubmed]
  8. Direct selection of mutants influencing gene conversion in the yeast Schizosaccharomyces pombe. Thuriaux, P. Mol. Gen. Genet. (1985) [Pubmed]
  9. Identification of the structural gene and nonsense alleles for adenylate cyclase in Saccharomyces cerevisiae. Matsumoto, K., Uno, I., Ishikawa, T. J. Bacteriol. (1984) [Pubmed]
  10. Genetic mapping of arg1 and arg8 in Saccharomyces cerevisiae by trisomic analysis combined with interallelic complementation. Hilger, F., Mortimer, R.K. J. Bacteriol. (1980) [Pubmed]
  11. Mutations preventing expression of sup3 tRNASer nonsense suppressors of Schizosaccharomyces pombe. Pearson, D., Willis, I., Hottinger, H., Bell, J., Kumar, A., Leupold, U., Söll, D. Mol. Cell. Biol. (1985) [Pubmed]
 
WikiGenes - Universities