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Gene Review

GDH1  -  glutamate dehydrogenase (NADP(+)) GDH1

Saccharomyces cerevisiae S288c

Synonyms: DHE4, NADP-GDH 1, NADP-dependent glutamate dehydrogenase 1, NADP-specific glutamate dehydrogenase 1, URE1, ...
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Disease relevance of GDH1

  • One of the cloned fragments was sequenced, and the deduced amino acid (aa) sequence of the yeast NADP-GDH is 64% homologous to N. crassa, 51% to Escherichia coli and 24% to bovine NADP-GDHs [1].

High impact information on GDH1


Biological context of GDH1

  • The HAP complex is known to regulate expression of several genes involved in carbon metabolism; its role in the control of GDH1 gene expression, therefore, provides evidence for a cross-pathway regulation between carbon and nitrogen metabolisms [5].
  • A computer search for similarities between the GDH1 nucleotide sequence and the complete yeast genome was carried out [6].
  • Regulation of GDH1 was not maintained when the gene was present on a multicopy plasmid [4].
  • Analysis of the nucleotide sequence of the GDH1 homologues from Saccharomyces bayanus strain CBS 380T and S. pastorianus strains showed that they share an almost identical sequence, SuGDH1*, which is a diverged form of the SuGDH1 from the type strain of the former species S. uvarum, considered as synonym of S. bayanus [7].
  • A yeast shuttle vector (CV13) carrying either to the cloned fragments complements the gdh- strain of S. cerevisiae and directs substantial overproduction of NADP-GDH [1].

Anatomical context of GDH1

  • Following an ammonia pulse, the expression of GAP1, PUT4 and GDH1 decreased while the intracellular glutamine concentration remained constant, both in the cytoplasm and in the vacuole [8].
  • Third, the steady-state level of GDH1 mRNA decreases about threefold in leu3 null cells [2].

Associations of GDH1 with chemical compounds


Other interactions of GDH1

  • GLT1-lacZ fusions were prepared and GLT1 expression was determined in a GDH1 wild-type strain and in a gdh1 mutant derivative grown in the presence of various nitrogen sources [12].
  • Here, we have cloned and characterized Hebeloma cylindrosporum AMT1, GLNA and GDHA genes, which encode a third ammonium transporter, a glutamine synthetase and an NADP-dependent glutamate dehydrogenase respectively [13].
  • In the present study, a metabolic flux model was constructed for two recombinant S. cerevisiae strains: TMB3001 and CPB.CR4 which in addition to xylose metabolism have a modulated redox metabolism, i.e. ammonia assimilation was shifted from being NADPH to NADH dependent by deletion of gdh1 and over-expression of GDH2 [14].
  • Enzymes subject to carbon catabolite inactivation and inactivation of NADP-dependent glutamate dehydrogenase, processes which were proposed to be of proteolytic nature, are not affected by the absence of proteinase A. However, protein degradation under sporulation conditions is about 30% reduced in proteinase A mutant cells [15].

Analytical, diagnostic and therapeutic context of GDH1


  1. Nucleotide sequence of the GDH gene coding for the NADP-specific glutamate dehydrogenase of Saccharomyces cerevisiae. Nagasu, T., Hall, B.D. Gene (1985) [Pubmed]
  2. The Saccharomyces cerevisiae Leu3 protein activates expression of GDH1, a key gene in nitrogen assimilation. Hu, Y., Cooper, T.G., Kohlhaw, G.B. Mol. Cell. Biol. (1995) [Pubmed]
  3. NADP-glutamate dehydrogenase isoenzymes of Saccharomyces cerevisiae. Purification, kinetic properties, and physiological roles. DeLuna, A., Avendano, A., Riego, L., Gonzalez, A. J. Biol. Chem. (2001) [Pubmed]
  4. Nucleotide sequence of yeast GDH1 encoding nicotinamide adenine dinucleotide phosphate-dependent glutamate dehydrogenase. Moye, W.S., Amuro, N., Rao, J.K., Zalkin, H. J. Biol. Chem. (1985) [Pubmed]
  5. The CCAAT box-binding factor stimulates ammonium assimilation in Saccharomyces cerevisiae, defining a new cross-pathway regulation between nitrogen and carbon metabolisms. Dang, V.D., Bohn, C., Bolotin-Fukuhara, M., Daignan-Fornier, B. J. Bacteriol. (1996) [Pubmed]
  6. GDH3 encodes a glutamate dehydrogenase isozyme, a previously unrecognized route for glutamate biosynthesis in Saccharomyces cerevisiae. Avendaño, A., Deluna, A., Olivera, H., Valenzuela, L., Gonzalez, A. J. Bacteriol. (1997) [Pubmed]
  7. Evolutionary relationships between the former species Saccharomyces uvarum and the hybrids Saccharomyces bayanus and Saccharomyces pastorianus; reinstatement of Saccharomyces uvarum (Beijerinck) as a distinct species. Nguyen, H.V., Gaillardin, C. FEMS Yeast Res. (2005) [Pubmed]
  8. Repression of nitrogen catabolic genes by ammonia and glutamine in nitrogen-limited continuous cultures of Saccharomyces cerevisiae. ter Schure, E.G., Silljé, H.H., Vermeulen, E.E., Kalhorn, J.W., Verkleij, A.J., Boonstra, J., Verrips, C.T. Microbiology (Reading, Engl.) (1998) [Pubmed]
  9. GDH1 expression is regulated by GLN3, GCN4, and HAP4 under respiratory growth. Riego, L., Avendaño, A., DeLuna, A., Rodríguez, E., González, A. Biochem. Biophys. Res. Commun. (2002) [Pubmed]
  10. Genome-wide transcriptional response of a Saccharomyces cerevisiae strain with an altered redox metabolism. Bro, C., Regenberg, B., Nielsen, J. Biotechnol. Bioeng. (2004) [Pubmed]
  11. Binding studies of NADPH to NADP-specific L-glutamate dehydrogenase from Saccharomyces cerevisiae. Venard, R., Jallon, J.M., Fourcade, A., Iwatsubo, M. Eur. J. Biochem. (1975) [Pubmed]
  12. Regulation of expression of GLT1, the gene encoding glutamate synthase in Saccharomyces cerevisiae. Valenzuela, L., Ballario, P., Aranda, C., Filetici, P., González, A. J. Bacteriol. (1998) [Pubmed]
  13. Molecular characterization, function and regulation of ammonium transporters (Amt) and ammonium-metabolizing enzymes (GS, NADP-GDH) in the ectomycorrhizal fungus Hebeloma cylindrosporum. Javelle, A., Morel, M., Rodríguez-Pastrana, B.R., Botton, B., André, B., Marini, A.M., Brun, A., Chalot, M. Mol. Microbiol. (2003) [Pubmed]
  14. Comparative metabolic network analysis of two xylose fermenting recombinant Saccharomyces cerevisiae strains. Grotkjaer, T., Christakopoulos, P., Nielsen, J., Olsson, L. Metab. Eng. (2005) [Pubmed]
  15. Analysis of proteinase A function in yeast. Mechler, B., Wolf, D.H. Eur. J. Biochem. (1981) [Pubmed]
  16. A NADP-glutamate dehydrogenase mutant of the petit-negative yeast Kluyveromyces lactis uses the glutamine synthetase-glutamate synthase pathway for glutamate biosynthesis. Valenzuela, L., Guzmán-León, S., Coria, R., Ramírez, J., Aranda, C., González, A. Microbiology (Reading, Engl.) (1995) [Pubmed]
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