High impact information on STH1

• Furthermore, in non-induced cells depleted for Sth1p/Nps1p or Swh3p, a nucleosome positioned over the TATA box of the CHA1 promoter is disrupted, an architectural change normally only observed during transcriptional induction [1].
• However, the NPS1 gene was found to have a distinct function from SNF2/GAM1 [2].
• We have cloned the gene NPS1 (nuclear protein of Saccharomyces) which encodes a nuclear protein of mol. wt 156 735 Daltons (1359 amino acids) essential for cell growth [2].
• In the predicted amino acid sequence of NPS1, sequences homologous to the catalytic domain of protein kinases were found [2].
• The mutant gene failed to rescue the growth defect caused by NPS1 disruption, suggesting that Lys792 is essential for the function of NPS1 [2].

Biological context of STH1

• A mutation in NPS1/STH1, an essential gene encoding a component of a novel chromatin-remodeling complex RSC, alters the chromatin structure of Saccharomyces cerevisiae centromeres [3].
• BACKGROUND: The NPS1/STH1 gene of Saccharomyces cerevisiae is essential for mitotic growth, especially for the progression through the G2/M phase [4].
• We attempted to address the function of NPS1 in meiosis [4].
• Nps1/Sth1p, a component of an essential chromatin-remodeling complex of Saccharomyces cerevisiae, is required for the maximal expression of early meiotic genes [4].
• CONCLUSION: The results suggest that Nps1p/RSC is required for the activation of gene expression at the initiation of meiosis [4].

Associations of STH1 with chemical compounds

• We isolated a temperature-sensitive mutant allele of NPS1 , nps1-105, and found that the mutation increases the sensitivity to thiabendazole (TBZ) [3].

Other interactions of STH1

• In addition, the expression of NPS1 itself increased transiently upon initiation of meiosis, before the appearance of the IME2 message but after that of IME1 [4].
• The Snf2 sequence that interacts with Swh3 was mapped to a region conserved in Sth1 [5].
• Interaction of a Swi3 homolog with Sth1 provides evidence for a Swi/Snf-related complex with an essential function in Saccharomyces cerevisiae [5].
• Both sth1 and sfh1 mutants exhibit altered centromeric and centromere-proximal chromatin structure and increased missegregation of authentic chromosomes [6].

References