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POB3  -  FACT complex subunit POB3

Saccharomyces cerevisiae S288c

Synonyms: Facilitates chromatin transcription complex subunit POB3, YML069W
 
 
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High impact information on POB3

  • Spt16 functions as a component of the yeast CP (Cdc68/Pob3) and mammalian FACT (facilitates chromatin transcription) complexes, which are involved in transcription elongation and DNA replication [1].
  • The previously uncharacterized Pob3 sequence has significant amino acid sequence similarity with an HMG1-like protein from vertebrates [2].
  • Mutations in POB3, but not in NHP6A/B, also display strong synthetic defects with hir/hpc mutations [3].
  • Mutation of the S phase checkpoint gene MEC1 caused pob3 mutants to lose viability rapidly under restrictive conditions, revealing defects in a process monitored by Mec1 [4].
  • Direct examination of DNA contents by flow cytometry showed that S phase onset and progression were delayed when POB3 was mutated [4].
 

Biological context of POB3

 

Associations of POB3 with chemical compounds

  • Nhp6 interacts with Spt16/Pob3, the yeast equivalent of the FACT elongation complex, consistent with nhp6ab cells being extremely sensitive to 6-azauracil (6-AU) [8].
 

Other interactions of POB3

  • These results suggest that Spt16-Pob3 and Nhp6 cooperate to function as a novel nucleosome reorganizing factor [5].
  • Spt16, Pob3, and Pol1 proteins were all found to localize to the nucleus in S. cerevisiae [9].

References

  1. The something about silencing protein, Sas3, is the catalytic subunit of NuA3, a yTAF(II)30-containing HAT complex that interacts with the Spt16 subunit of the yeast CP (Cdc68/Pob3)-FACT complex. John, S., Howe, L., Tafrov, S.T., Grant, P.A., Sternglanz, R., Workman, J.L. Genes Dev. (2000) [Pubmed]
  2. The Saccharomyces cerevisiae DNA polymerase alpha catalytic subunit interacts with Cdc68/Spt16 and with Pob3, a protein similar to an HMG1-like protein. Wittmeyer, J., Formosa, T. Mol. Cell. Biol. (1997) [Pubmed]
  3. Defects in SPT16 or POB3 (yFACT) in Saccharomyces cerevisiae cause dependence on the Hir/Hpc pathway: polymerase passage may degrade chromatin structure. Formosa, T., Ruone, S., Adams, M.D., Olsen, A.E., Eriksson, P., Yu, Y., Rhoades, A.R., Kaufman, P.D., Stillman, D.J. Genetics (2002) [Pubmed]
  4. POB3 is required for both transcription and replication in the yeast Saccharomyces cerevisiae. Schlesinger, M.B., Formosa, T. Genetics (2000) [Pubmed]
  5. Spt16-Pob3 and the HMG protein Nhp6 combine to form the nucleosome-binding factor SPN. Formosa, T., Eriksson, P., Wittmeyer, J., Ginn, J., Yu, Y., Stillman, D.J. EMBO J. (2001) [Pubmed]
  6. A bipartite yeast SSRP1 analog comprised of Pob3 and Nhp6 proteins modulates transcription. Brewster, N.K., Johnston, G.C., Singer, R.A. Mol. Cell. Biol. (2001) [Pubmed]
  7. Characterization of the CP complex, an abundant dimer of Cdc68 and Pob3 proteins that regulates yeast transcriptional activation and chromatin repression. Brewster, N.K., Johnston, G.C., Singer, R.A. J. Biol. Chem. (1998) [Pubmed]
  8. TATA-binding protein mutants that are lethal in the absence of the Nhp6 high-mobility-group protein. Eriksson, P., Biswas, D., Yu, Y., Stewart, J.M., Stillman, D.J. Mol. Cell. Biol. (2004) [Pubmed]
  9. Spt16 and Pob3 of Saccharomyces cerevisiae form an essential, abundant heterodimer that is nuclear, chromatin-associated, and copurifies with DNA polymerase alpha. Wittmeyer, J., Joss, L., Formosa, T. Biochemistry (1999) [Pubmed]
 
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