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RPS17A  -  ribosomal 40S subunit protein S17A

Saccharomyces cerevisiae S288c

Synonyms: 40S ribosomal protein S17-A, RP51A, RPL51A, YML024W
 
 
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High impact information on RPS17A

  • Complex 2, formed on TEF2 or RP51A probes at higher protein concentrations, corresponded to an extended footprint of 35-40 bp [1].
  • Complex 1, formed on TEF1, TEF2 and RP51A 5'-flanking region, was correlated with the protection of a 25-bp sequence [1].
  • Primer extension analysis of the 5' terminus of ribosomal protein 51A (RP51A) mRNA showed that about 30% of the poly(A)-deficient molecules of the xrn1 cells are slightly shorter at the 5' end [2].
  • We altered the RP51 gene dose by creating deletions of the RP51A or RP51B genes or both [3].
  • Deletions were made in the RP51A-coding portion of the fusion gene [4].
 

Biological context of RPS17A

  • Thus, a regulatory site (or sites) lies in the protein-coding region of RP51A [4].
  • The cloned genes, in combination with the one-step gene disruption techniques of Rothstein (R. J. Rothstein, Methods Enzymol. 101:202-211, 1983), were used to generate haploid strains containing mutations in the RP51A or RP51B genes or in both [5].
 

Anatomical context of RPS17A

  • In the absence of the RP51A gene, the fusion protein is predominantly cytoplasmic and associated with polysomes, whereas in the presence of RP51A, the fusion protein is predominantly nuclear, and none is associated with polysomes [4].
 

Other interactions of RPS17A

  • The results of Northern blot and primer extension experiments indicate that strains with a wild-type copy of the RP51B gene and a mutant (or deleted) RP51A gene grow slowly because of an insufficient amount of RP51 mRNA [5].
  • The intron of the yeast RP51A gene was cloned with precision using the polymerase chain reaction (PCR) amplification method, and then inserted into several different positions of the yeast URA3 gene as well as the PGK-lacZ fusion gene without introduction of additional exon sequences [6].

References

  1. A general upstream binding factor for genes of the yeast translational apparatus. Huet, J., Cottrelle, P., Cool, M., Vignais, M.L., Thiele, D., Marck, C., Buhler, J.M., Sentenac, A., Fromageot, P. EMBO J. (1985) [Pubmed]
  2. Yeast cells lacking 5'-->3' exoribonuclease 1 contain mRNA species that are poly(A) deficient and partially lack the 5' cap structure. Hsu, C.L., Stevens, A. Mol. Cell. Biol. (1993) [Pubmed]
  3. Effect of RP51 gene dosage alterations on ribosome synthesis in Saccharomyces cerevisiae. Abovich, N., Gritz, L., Tung, L., Rosbash, M. Mol. Cell. Biol. (1985) [Pubmed]
  4. Posttranscriptional regulation and assembly into ribosomes of a Saccharomyces cerevisiae ribosomal protein-beta-galactosidase fusion. Gritz, L., Abovich, N., Teem, J.L., Rosbash, M. Mol. Cell. Biol. (1985) [Pubmed]
  5. Two genes for ribosomal protein 51 of Saccharomyces cerevisiae complement and contribute to the ribosomes. Abovich, N., Rosbash, M. Mol. Cell. Biol. (1984) [Pubmed]
  6. Effect of artificially inserted intron on gene expression in Saccharomyces cerevisiae. Yoshimatsu, T., Nagawa, F. DNA Cell Biol. (1994) [Pubmed]
 
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